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Human stem cell technology and biology : a research guide and laboratory manual / edited by Gary S. Stein ... [et al.]. — Hoboken, N.J. : Wiley ; Chichester : John Wiley [distributor], c2011. – (59.41/H918h) |
Contents
Contents
Foreword ix
Preface xi
Acknowledgments xiii
Editors and Contributors xv
SECTION I INTRODUCTION
1 Introduction to Pluripotent Stem Cells: Biology and Applications
2 Researching and Obtaining Established Stem Cell Lines 9
SECTION II LABORATORY GUIDE FOR HUMAN STEM CELL CULTURE: PLURIPOTENT STEM CELL CULTURE
3 Basics of Cell Culture 19
4 The Stem Cell Laboratory 33
5 Reagent Preparation 41
6 Preparation of Mouse Embryonic Fibroblasts for Culture of Human Embryonic Stem Cells 59
7 Thawing and Seeding of Frozen Mouse Embryonic Fibroblasts
8 Thawing， Seeding， and Changing the Medium of Human Embryonic Stem Cells 95
9 Passaging of Human Embryonic Stem Cells on Inactivated Mouse Embryonic Fibroblast Plates 105
10 Harvesting Human Embryonic Stem Cells for Cryopreservation 119
11 Human Embryonic Stem Cell Culture on BD Matrigel with mTeSR1 Medium 129
SECTION III LABORATORY GUIDE FOR HUMAN STEM CELL CULTURE: CHARACTERIZATION OF PLURIPOTENT STEM CELLS
12 Defining Pluripotency 141
13 Characterization of Human Embryonic Stem Cells by Immunofluorescence Microscopy
14 Preparation of Human Embryonic Stem Cell Samples for Flow Cytometry 159
15 Characterization of Human Embryonic Stem Cell Gene Expression Using Reverse Transcription Polymerase Chain Reaction 167
16 Determining Pluripotency of Human Embryonic Stem Cells: Embryoid Body Formation
17 Characterization of Human Embryonic Stem Cells by Cytogenetics: Karyotyping and Fluorescence In Situ Hybridization 199
18 High-Resolution Chromatin Immunoprecipitation Assay 211
19 Assaying Pluripotency Via Teratoma Formation 217
SECTION IV PERSPECTIVES IN HUMAN STEM CELL TECHNOLOGIES
20 Genomic Analysis of Pluripotent Stem Cells 237
21 Proteomic Analysis of Human Pluripotent Cells 245
22 Bioinformatics Strategies for Understanding Gene Expression in Human Pluripotent Cells 257
23 Epigenetic Analysis of Pluripotent Cells 273
24 Differentiation of Pluripotent Stem Cells: An Overview 289
25 Cellular Reprogramming: Current Technology， Perspectives， and Generation of Induced Pluripotent Cells 297
SECTION V APPLICATIONS OF HUMAN EMBRYONIC STEM CELLS
26 Human Pluripotent Cells: The Biology of Pluripotency 313
27 Human Pluripotent Cells for Regenerative Medicine: Potential Applications for Regenerative Medicine 327
28 Therapeutic Applications of Human Embryonic Stem Cells 339
29 Human Stem Cells for Drug Screening， Toxicity， Specificity， and Off-Target Effects 353
30 Interfacing Stem Cells with Gene Therapy 367
31 Tissue Engineering for Stem Cell Mediated Regenerative Medicine 377
Glossary 401
Index 413
