RNAi and plant gene function analysis : methods and protocols / edited by Hiroaki Kodama, Atsushi Komamine. — New York : Springer, c2011. – (58.17/M592/v.744)

Contents

    Contents
    
    Preface
    Contributors ix
    1. Overview of Plant RNAi
    2. Caveat of RNAi in Plants: The Off Target Effect
    3. Plant Gateway Vectors for RNAi as a Tool for Functional Genomic Studies 27
    4. Heat-Inducible RNAi for Gene Functional Analysis in Plants 37
    5. Gene Function Analysis by Artificial MicroRNAs in Physcomitrella patens 57
    6. Virus-Induced Gene Silencing in Ornamental Plants 81
    7. Local RNA Silencing Mediated by Agroinfiltration 97
    8. Direct Transfer of Synthetic Double-Stranded RNA into Protoplasts of Arabidopsis thaliana 109
    9. Detection of Long and Short Double-Stranded RNAs 129
    10. Quantitative Stem-Loop RT-PCR for Detection of MicroRNAs 145
    11. Large-Scale Sequencing of Plant Small RNAs 159
    12. Computational Prediction of Plant miRNA Targets 175
    13. Bisulfite Sequencing for Cytosine-Methylation Analysis in Plants 187
    14. Using Nuclear Run-On Transcription Assays in RNAi Studies 199
    15. Proteomic Analysis of RNA Interference Induced Knockdown Plant
    16. Comparative Analysis of Phosphoprotein Expression Using 2D-DIGE 225
    Index 235