MicroRNAs in development : methods and protocols / edited by Tamas Dalmay. — Totowa, N.J. : Humana, 2011. – (58.17/M592/v.732)

Contents

    CONTENTS
    
    Preface
    Contributors
    1 In Situ Detection of microRNAs in Animals
    2 Detection of microRNAs in Plants by In Situ Hybridisation
    3 Detecting sRNAs by Northern Blotting
    4 Profiling MicroRNAs by Real-Time PCR 39
    5 Detection of Small RNAs and MicroRNAs Using Deep Sequencing Technology 55
    6 Detection of MicroRNAs in Prostate Cancer Cells by MicroRNA Array 69
    7 MicroRNA Knock Down by Cholesterol-Conjugated Antisense Oligos in Mouse Organ Culture 89
    8 Protocols for Use of Homologous Recombination Gene Targeting to Produce MicroRNA Mutants in Drosophila 99
    9 Engineering Elements for Gene Silencing: The Artificial MicroRNAs Technology
    10 Mimicry Technology: Suppressing Small RNA Activity in Plants 131
    11 Experimental Validation of MicroRNA Targets Using a Luciferase Reporter System
    12 Experimental Identification of MicroRNA Targets by Immunoprecipitation of Argonaute Protein Complexes 153
    13 Comprehensive Identification of miRNA Target Sites in Live Animals 169
    14 Target Validation of Plant microRNAs
    15 A High-Throughput Sequencing-Based Methodology to Identify All Uncapped and Cleaved RNA Molecules in Eukaryotic Genomes 209
    Index