The plant cell wall : methods and protocols / edited by Zoe A. Popper. — [Totowa, N.J.] : Humana, c2011. – (58.17/M592/v.715)

Contents

    Contents
    
    1 Plant Tissue Cultures 1
    2 Computerized Molecular Modeling of Carbohydrates 21
    3 Oligosaccharide Mass Profiling (OLIMP) of Cell Wall Polysaccharides by MALDI-TOF/MS
    4 High-Voltage Paper Electrophoresis (HVPE) of Cell-Wall Building Blocks and Their Metabolic Precursors
    5 Carbohydrate Gel Electrophoresis 81
    6 Capillary Electrophoresis with Detection by Laser-Induced Fluorescence 93
    8 Screening and Characterization of Plant Cell Walls Using Carbohydrate Microarrays 115
    9 Electron Tomography and Immunogold Labelling as Tools to Analyse De Novo Assembly of Plant Cell Walls
    10 Analysing Cellulose Biosynthesis with Confocal Microscopy 141
    11 Visual Mapping of Cell Wall Biosynthesis 153
    12 Atomic Force Microscopy of Plant Cell Walls
    13 Using Solid-State ]3C NMR Spectroscopy to Study the Molecular Organisation of Primary Plant Cell Walls 179
    14 Formation of Cellulose-Based Composites with Hemicelluloses and Pectins Using Gluconacetobacter Fermentation 197
    15 Structural Proteins of the Primary Cell Wall: Extraction， Purification， and Analysis
    16 New Insights into the Control of Cell Growth 221
    17 Extraction and Detection of Arabinogalactan Proteins 245
    18 Characterization of the Plant Cell Wall Proteome Using High-Throughput Screens
    19 Knocking Out the Wall: Protocols for Gene Targeting in Physcomitrella patens
    20 Measuring In Vitro Extensibility of Growing Plant Cell Walls 291
    Index 305