Plant immunity : methods and protocols / edited by John M. McDowell. — [New York] : Humana, c2011. – (58.17/M592/v.712)

Contents

    Contents
    
    Preface
    Contributors
    1 Studying NB-LRR Immune Receptor Localization by Agroinfiltration Transient Expression
    2 Fragment Complementation and Co-immunoprecipitation Assays for Understanding R Protein Structure and Function
    3 Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag
    4 Biochemical Purification of Native immune Protein Complexes
    5 Chromatin Immunoprecipitation to Identify Global Targets of WRKY Transcription Factor Family Members Involved in Plant Immunity
    6 Dose-Response to and Systemic Movement of Dexamethasone in the GVG-Inducible Transgene System in Arabidopsis
    7 Quantifying Alternatively Spliced mRNA via Capillary Electrophoresis
    8 Constructing Haustorium-Specific cDNA Libraries from Rust Fungi
    9 Microaspiration of Esophageal Gland Cells and cDNA Library Construction for Identifying Parasitism Genes of Plant-Parasitic Nematodes
    10 Construction of Pseudomonas syringae pv. tomato DC3000 Mutant and Polymutant Strains
    11 A Straightforward Protocol for Electro-transformation of Phytophthora capsici Zoospores
    12 Propagation， Storage， and Assays with Hyaloperonospora arabidopsidis: A Model Oomycete Pathogen of Arabidopsis. 137
    13 Assaying Effector Function in Planta Using Double-Barreled Particle Bombardment
    14 Assays for Effector-Mediated Suppression of Programmed Cell Death in Yeast
    15 Purification of Effector-Target Protein Complexes via Transient Expression in Nicotiana benthamiana
    16 Imaging Fluorescently Tagged Phytophthora Effector Proteins Inside Infected Plant Tissue
    17 Immunolocalization of Pathogen Effectors
    18 Laser Capture Microdissection of Nematode Feeding Cells
    19 Laser Microdissection of Plant-Fungus Interaction Sites and Isolation of RNA for Downstream Expression Profiling
    20 Global Expression Profiling of RNA from Laser Microdissected Cells at Fungal-Plant Interaction Sites
    21 Visualizing Cellular Dynamics in Plant-Microbe Interactions Using Fluorescent-Tagged Proteins 283
    Index 293