Embryonic stem cell therapy for osteo-degenerative diseases : methods and protocols / edited by Nicole I. zur Nieden. — New York : Humana, c2011. – (58.17/M592/v.690)

Contents

    CONTENTS
    
    Preface
    Contributors
    1 Embryonic Stem Cells for Osteo-Degenerative Diseases
    2 Methods for Culturing Mouse and Human Embryonic Stem Cells
    3 Serum-Free and Feeder-Free Culture Conditions for Human Embryonic Stem Cells
    4 Functional Assays for Human Embryonic Stem Cell Pluripotency
    5 Using Cadherin Expression to Assess Spontaneous Differentiation of Embryonic Stem Cells
    6 Generation of Human Embryonic Stem Cells Carrying Lineage Specific Reporters
    7 Manipulations of MicroRNA in Human Pluripotent Stem Cells and Their Derivatives
    8 Large-Scale Expansion of Mouse Embryonic Stem Cells on Microcarriers 121
    9 Embryoid Body Formation: Recent Advances in Automated Bioreactor Technology
    10 Methods for Embryoid Body Formation: The Microwell Approach
    11 Human Embryonic Stern Cell-Derived Mesenchymal Progenitors: An Overview
    12 Derivation of Mesenchymal Stem Cells from Human Embryonic Stem Cells
    13 Differentiation of Human Embryonic Stem Cells into Mesenchymal Stem Cells by the "Raclure" Method
    14 Improved Media Compositions for the Differentiation of Embryonic Stem Cells into Osteoblasts and Chondrocytes 195
    15 Differentiation of Mouse Embryonic Stem Cells in Self-Assembling Peptide Scaffolds 217
    16 Methods for Investigation of Osteoclastogenesis Using Mouse Embryonic Stem Cells
    17 Absorption-Based Assays for the Analysis of Osteogenic and Chondrogenic Yield
    18 Identification of Osteoclasts in Culture
    19 Analysis of Glycosaminoglycans in Stem Cell Glycomics
    20 Drill Hole Defects: Induction， Imaging， and Analysis in the Rodent 301
    21 Measurement and Illustration of Immune Interaction After Stem Cell Transplantation
    Index