Adipose-derived stem cells : methods and protocols / edited by Jeffrey M. Gimble, Bruce A. Bunnell. — New York, N.Y. : Humana, c2011. – (58.17/M592/v.702)

Contents

    CONTENTS
    
    Preface
    Contributors
    PART I ISOLATION METHODS
    1 Isolation and Culture of Rhesus Adipose-Derived Stem Cells
    2 Isolation of Human Adipose-Derived Stein Cells from Lipoaspirates
    3 Isolation of Murine Adipose-Derived Stem Cells
    4 In Vitro Adult Rat Adipose Tissue-Derived Stromal Cell Isolation and Differentiation
    5 In Vitro Adult Canine Adipose Tissue-Derived Stromal Cell Growth Characteristics
    6 Adipogenic Differentiation of Adult Equine Mesenchymal Stromal Cells
    7 Isolation and Culture of Porcine Adipose Tissue-Derived Somatic Stem Cells
    8 Automated Isolation and Processing of Adipose-Derived Stem and Regenerative Cells
    PART II PHENOTYPING METHODS
    9 Methods for the Purification and Characterization of Human Adipose-Derived Stem Cells
    10 Characterization of Human Adipose-Derived Stem Cells Using Flow Cytometry
    11 Evaluation of Cellular and Humoral Immune Responses to Allogeneic Adipose-Derived Stem/Stromal Cells
    12 Adipose-Derived Stromal Cells: Cytokine Expression and Immune Cell Contaminants
    13 Gel-Based and Gel-Free Proteomic Technologies 163
    PART III DIFFERENTIATION METHODS: IN VITRO AND IN VIVO APPLICATIONS
    14 Adipogenic Differentiation of Adipose-Derived Stem Cells 193
    15 Three-Dimensional Culture Systems to Induce Chondrogenesis of Adipose-Derived Stem Cells 201
    16 Neural Differentiation of Human Adipose Tissue-Derived Stem Cells 219
    17 Osteogenic Differentiation Strategies for Adipose-Derived Mesenchymal Stem Cells 233
    18 Generation of Adipose Stromal Cell-Derived Hepatic Cells 249
    19 Adipose Stem Cell Differentiation into Smooth Muscle Cells 261
    20 Endothelial and Cardiac Regeneration from Adipose Tissues 269
    21 Epithelial Differentiation of Human Adipose-Derived Stem Cells 289
    22 Screening for Epigenetic Target Genes that Enhance Reprogramming Using Lentiviral-Delivered shRNA 299
    PART IV BIOENGINEERING， MANUFACTURING~ AND CLINICAL TECHNOLOGIES
    23 Adipogenic Differentiation of Human Adipose-Derived Stem Cells on 3D Silk Scaffolds
    24 A First Approach for the Production of Human Adipose Tissue-Derived Stromal Cells for Therapeutic Use 331
    25 Viral Transduction of Adipose-Derived Stem Cells 345
    26 Use of Adipose-Derived Stem Cells in High-Throughput Screening to Identify Modulators of Lipogenesis 359
    27 Preservation Protocols for Human Adipose Tissue-Derived Adult Stem Cells 369
    28 Soft Tissue Reconstruction
    29 Methods for Analyzing MicroRNA Expression and Function During Osteogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells
    30 The Generation and the Manipulation of Human Multipotent Adipose-Derived Stem Cells
    31 Cell Sheet Technology for Tissue Engineering: The Self-Assembly Approach Using Adipose-Derived Stromal Cells
    32 Differentiation of Adipose-Derived Stem Cells for Tendon Repair
    33 Adipose-Derived Stem Cells for Skin Regeneration
    34 Adipose-Derived Stem Cells for Periodontal Tissue Regeneration
    Index