Plant reverse genetics : methods and protocols / edited by Andy Pereira. — New York : Humana, c2011. – (58.17/M592/v.678)

Contents

    Contents
    
    Preface
    Contributors.
    1 Analysis of High-Throughput Sequencing Data
    2 Identification of Plant microRNAs Using Expressed Sequence Tag Analysis
    3 Microarray Data Analysis 27
    4 Setting Up Reverse Transcription Quantitative-PCR Experiments 45
    5 Virus-Induced Gene Silencing in Nicotiana benthamiana and Other Plant Species
    6 Agroinoculation and Agroinfiltration: Simple Tools for Complex Gene Function Analyses 65
    7 Full-Length cDNA Overexpressor Gene Hunting System (FOX Hunting System)
    8 Activation Tagging with En/Spm-I/dSpm Transposons in Arabidopsis
    9 Activation Tagging and Insertional Mutagenesis in Barley
    10 Methods for Rice Phenomics Studies
    11 Development of an Efficient Inverse PCR Method for Isolating Gene Tags from T-DNA Insertional Mutants in Rice
    12 Transposon Insertional Mutagenesis in Rice
    13 Reverse Genetics in Medicago truncatula Using Tnt1 Insertion Mutants
    14 Screening Arabidopsis Genotypes for Drought Stress Resistance
    15 Protein Tagging for Chromatin Immunoprecipitation from Arabidopsis
    16 Yeast One-Hybrid Screens for Detection of Transcription Factor DNA Interactions
    17 Plant Metabolomics by GC-MS and Differential Analysis 229
    18 Gramene Database: A Hub for Comparative Plant Genomics 247
    Index 277