RNA therapeutics : function, design, and delivery / edited by Mouldy Sioud. — Totowa, N.J. : Humana, 2010. – (58.17/M592/v.629)

Contents

    Contents
    
    Preface v
    Contributors
    1. RNA Modifications: A Mechanism that Modulates Gene Expression
    2. Quantitative Analysis of RNA Modifications 21
    3. Advances ill ILNA Sensing by the Immune System: Separation of siRNA Unwanted Effects from RNA Interference 33
    4. Progress in siRNA Delivery Using Multifunctional Nanoparticles 53
    5. Optimized Protocols for siRNA Delivery into Monocytes and Dendritic Cells
    6. Systemic Delivery of Synthetic siRNAs 85
    7. What Are the Key Targeted Delivery Technologies of siRNA Now? 91
    8. Using OligoWalk to Identify Efficient siRNA Sequences 107
    9. Jet-Injection of Short Hairpin RNA-Encoding Vectors into Tumor Cells
    10. Short Hairpin RNA (shRNA): Design， Delivery， and Assessment of Gene Knockdown
    11. Effective Pol III-Expressed Long Hairpin RNAs Targeted to Multiple Unique Sites of HIV-I 157
    12. Functional Studies on RNA-Transfected Cell Microarrays 173
    13. Transfection Microarrays for High-Throughput Phenotypic Screening of Genes Involved in Cell Migration
    14. Intron-Mediated RNA Interference， Intronic MicroRNAs， and Applications
    15. Inhibition of the microRNA Pathway in Zebrafish by siRNA 237
    16. Profiling of miRNA Expression and Prediction of Target Genes 255
    17. Small RNA Cloning
    18. In Situ Hybridization Detection of microRNAs 285
    19. Detection and Quantitative Analysis of Small RNAs by PCR 293
    20. In Vivo Reprogramming of Human Telomerase Reverse Transcriptase (hTERT) by Trans-Splicing Ribozyme to Target Tumor Cells 305
    21. Design and Function of Triplex Hairpin Ribozymes 321
    22. Catalytic M1GS RNA as an Antiviral Agent in Animals 337
    23. Using Live Cells to Generate Aptamers for Cancer Study 353
    24. Primer-Free Aptamer Selection Using a 1Lmdom DNA Library 367
    25. Development of TLR7/8 Small RNA Antagonists 385
    26. Modulation of Dendritic Cell Maturation and Function by siRNA-Bearing 5'-Triphosphate
    27. Immunotherapy of Cancer with Dendritic Cells Loaded with Tumor Antigens and Activated Through mRNA Electroporation
    28. Non-MHC-Dependent Redirected T Cells Against Tumor Cells 451
    29. Overcoming Self-Tolerance to Tumour Cells
    30. Recent Advances in Hematopoietic Stem Cell Transplantation and Perspectives of RNAi Applications
    Subject Index