RNAi and microRNA-mediated gene regulation in stem cells : methods, protocols, and applications / edited by, Baohong Zhang and Edmund J. Stellwag. — New York, N.Y. : Humana, c2010. – (58.17/M592/v.650)

Contents

    Contents
    
    Preface
    Contributors
    SECTION I INTRODUCTION
    1. RNAi in Stem Cells: Current Status and Future Perspectives
    2. A Brief Introduction to RNAi and MicroRNAs in Stem Cells
    SECTION II RNA INTERFERENCE
    3. Forward RNAi Screens in Human Stem Cells
    4. A Recessive Genetic Screen for Components of the RNA Interference Pathway in Mouse Embryonic Stem Cells
    5. Construction and Application of Random dsRNA Interference Library for Functional Genetic Screens in Embryonic Stem Cells
    6. Establishing Efficient siRNA Knockdown in Stem Cells Using Fluorescent Oligonucleotides
    7. Loss-of-Function Studies in Mouse Embryonic Stem Cells Using the pHYPER shRNA Plasmid Vector
    8. Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNAi Directed Against Transcription Factor L3/Lhx8
    9. Silencing of Rho-GDIγ by RNAi Promotes the Differentiation of Neural Stem Cells
    10. RNAi Knockdown of Redox Signaling Protein Apel in the Differentiation of Mouse Embryonic Stem Cells
    11. Proteins Involved in Cell Migration from Glioblastoma Neurospheres Analyzed by Overexpression and siRNA-Mediated Knock-Down
    12. An Efficient Transfection Method for Mouse Embryonic Stem Cells
    13. Semi-quantitative Analysis of Transient Single-Cell Gene Expression in Embryonic Stem Cells by Femtoinjection
    SECTION III MicroRNAs
    14. Preparation and Analysis of MicroRNA Libraries Using the Illumina Massively Parallel Sequencing Technology
    15. Assessing In Vivo MicroRNA Function in the Germline Stem Cells of the Drosophila Ovary
    16. Monitoring MicroRNA Expression During Embryonic Stem-Cell Differentiation Using Quantitative Real-Time PCR (qRT-PCR)
    17. Engineering Human Mesenchymal Stem Cells to Release Adenosine Using miRNA Technology
    18. Efficient Gene Knockdowns in Mouse Embryonic Stem Cells Using MicroRNA-Based shRNAs
    Subject Index