Essential ion channel methods / edited by P. Michael Conn. — Amsterdam : Elsevier/Academic Press, 2010. – (58.1574/E78)

Contents

    CONTENTS
    
    Contributors
    Preface
    PART I Assembly
    1. Assembly of Ion Channels
    PART II Genetics
    2. Identification of Ion Channel-Associated Proteins Using the Yeast Two-Hybrid System
    PART III Electrophysiology
    3. Patch-Clamp Studies of Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channel
    4. Tight-Seal， Whole-Cell Patch Clamping of C. elegans Neurons.
    5. Gating Currents
    6. Determining Ion Channel Permeation Properties
    PART IV Expression Systems
    7. Expression of Ligand-Gated Ion Channels Using Semliki Forest Virus and Baculovirus
    8. Recombinant Adenovirus-Mediated Expression in the Nervous System of Genes Coding for Ion Channels and Other Molecules Involved in Synaptic Function
    9. Transient Expression of Heteromeric Ion Channels
    PART V Model Simulations
    10. Computer Simulations and Modeling of Ion Channels
    PART VI Physical
    11. Fluorescence Techniques for Measuring Ion Channel Activity
    12. Ligand Binding Methods for Analysis of Ion Channel Structure and Function
    13. Three-Dimensional Structure of Membrane Proteins Determined by Two-Dimensional Crystallization， Electron Cryomicroscopy， and Image Analysis
    14. Voltage-Clamp Biosensors for Capillary Electrophoresis
    15. Ion Channels as Tools to Monitor Lipid Bilayer-Membrane Protein Interactions Gramicidin Channels as Molecular Force Transducers
    PART VII Purification and Reconstitution
    16. Purification and Reconstitution of Epithehal Chloride Channel Cystic Fibrosis Transmembrane Conductance Regulator
    17. Reconstitution of Native and Cloned Channels into Planar Bilayers
    PART VIII Second Messengers and Biochemical Approaches
    18. Protein Phosphorylation of Ligand-Gated Ion Channels
    19. Analysis of Ion Channel Associated Proteins
    20. Secondary Messenger Regulation of Ion Channels/Plant Patch Clamping
    PART IX Special Channels
    21. ATP-Sensitive Potassium Channels
    22. Simplified Fast Pressure-Clamp Technique for Studying Mechanically Gated Channels
    23. Purification and Heterologous Expression of Inhibitory Glycine Receptors
    24. Functional Analyses of Aquaporin Water Channel Proteins
    PART X Toxins and Other Membrane Active Compounds
    25. Conus Peptides as Probes for Ion Channels
    26. Scorpion Toxins as Tools for Studying Potassium Channels
    27. Use of Planar Lipid Bilayer Membranes for Rapid Screening of Membrane-Active Compounds
    28. Antibodies to Ion Channels