Guide to research techniques in neuroscience / Matt Carter, Jennifer C. Shieh. — Amsterdam : Elsevier, c2010. – (59.5905/C324)

Contents

    CONTENTS
    
    Preface
    Foreword
    Introduction
    1. Whole Brain Imaging
    Structural Brain Imaging Techniques
    Functional Brain Imaging Techniques
    Functional Imaging Experimental Design and Analysis
    Conclusion
    Suggested Reading and References
    2. Animal Behavior
    Considerations for Choosing and Performing a Behavioral Assay
    Rodent Behavioral Paradigms
    Drosophila Behavioral Paradigms
    C. elegans Behavioral Paradigms
    Nonhuman Primate Behavioral Paradigms
    Conclusion
    Suggested Reading and References
    3. Stereotaxic Surgeries and In Vivo Techniques
    Stereotaxic Surgeries
    Implants for Long-Term Access to the Brain
    Measuring Neural Activity In Vivo
    Measuring Neurochemistry In Vivo
    Manipulating the Brain In Vivo
    Conclusion
    Suggested Reading and References
    4. Electrophysiology
    A Brief Review of the Electrical Properties of Neurons
    The Electrophysiology Rig
    Types of Electrophysiology Recordings
    Electrophysiology Tissue Preparations
    Methods of Manipulating Neurons During
    Electrophysiology Experiments
    Conclusion
    Suggested Reading and References
    5. Microscopy
    Essential Principles of Microscopy
    Light Microscopy
    Fluorescence Microscopy
    Electron Microscopy
    Preparing and Interpreting Microscopy Data
    Conclusion
    Suggested Reading and References
    6. Visualizing Nervous System Structure
    Tissue Preparation
    Visualizing Morphology
    Fluorescent Labels
    Chromogenic/Colorimetric Labels
    Radioactive Labels
    Gold Labels 157
    Visualizing Gene and Protein Expression 157
    Visualizing Circuitry
    Conclusion
    Suggested Reading and References
    7. Visualizing Nervous System Function
    Static Markers of Activity
    Visualizing Neural Activity
    Optically Manipulating Neural Activity
    Visualizing Protein Function
    Optically Manipulating Protein Function
    Conclusion
    Suggested Reading and References
    8. Identifying Genes and Proteins of Interest
    A Brief Review of Genes and Proteins
    Genetic Screens
    In Silico Screens
    Molecular Screens
    Conclusion
    Suggested Reading and References
    9. Molecular Cloning and Recombinant DNA Technology
    Isolating DNA Fragments
    Cloning DNA
    Purifying DNA
    Identifying DNA
    Conclusion
    Suggested Reading and References
    10. Gene Delivery Strategies
    Physical Gene Delivery
    Chemical Gene Delivery
    Viral Gene Delivery
    Conclusion
    Suggested Reading and References
    11. Making and Using Transgenic Organisms
    Transgenes
    The Transgenic Construct
    Binary Transgenic Systems
    Making Transgenic Organisms
    Conclusion
    Suggested Reading and References
    12. Manipulating Endogenous Genes
    Genetic Targeting
    Disrupting Gene Products
    Conclusion
    Suggested Reading and References
    13. Cell Culture Techniques
    Cell Culture Equipment and Reagents
    Immortalized Cell Lines
    Primary Cell and Tissue Culture
    Stem Cell Cultures
    Manipulating Cells in Culture
    Conclusion
    Suggested Reading and References
    14. Biochemical Assays and Intracellular Signaling
    Introduction to Signal Transduction and Intracellular Signaling
    Fundamental Tools for Studying Proteins
    Investigating Protein Expression
    Investigating Protein-Protein Interactions
    Investigating Posttranslational Modifications
    Investigating Protein-DNA Interactions
    Conclusion
    Suggested Reading and References
    Glossary
    Index