Gene cloning and DNA analysis : an introduction / T.A. Brown. — 6th ed. — Oxford : Wiley-Blackwell, 2010. – (58.1483/B881/6th ed.)

Contents

    Contents
    
    Preface to the Sixth Edition xvi
    Part I The basic principles of gene cloning and DNA analysis
    1.Why Gene Cloning and DNA Analysis are Important 3
    2.Vectors for Gene Cloning: Plasmids and Bacteriophages 13
    3.Purification of DNA from Living Cells 25
    4.Manipulation of Purified DNA 45
    5.Introduction of DNA into Living Cells 72
    6.Cloning Vectors for E. coli 88
    7.Cloning Vectors for Eukaryotes 105
    8.How to Obtain a Clone of a Specific Gene 126
    9.The Polymerase Chain Reaction 147
    Part II The applications of gene cloning and DNA analysis in biotechnology
    10.Sequencing Genes and Genomes 165
    11.Studying Gene Expression and Function 185
    12.Studying Genomes 207
    Part III The Applicatiions of gene Cloning and DNA Analysis in Biotechnology
    13.Production of Protein from Cloned Genes 225
    14.Gene Cloning and DNA Analysis in Medicine 245
    15.Gene Cloning and DNA Analysis in Agriculture 264
    16.Gene Cloning and DNA Analysis in Forensic Science and Archaeology 282
    Glossary 298
    Index 312
    Companion website available at www.wiley.com/go/brown/cloning