Introduction to proteomics : principles and applications / Nawin Mishra. — Oxford : Wiley, c2010. – (58.17421/M678)

Contents

    CONTENTS
    
    FOREWORD
    PREFACE
    ABOUT THE AUTHOR
    CHAPTER 1 HISTORICAL PERSPECTIVES
    1.1. Introduction to Proteomics / 3
    1.2. Proteome and Proteomics / 7
    1.3. Genetics of Proteins / 9
    1.4. Molecular Biology of Genes and Proteins / 20
    1.5. Protein Chemistry Before Proteomics / 24
    References / 34
    Further Reading / 37
    CHAPTER 2 PROTEOMICS--RELATION TO GENOMICS， BIOINFORMATICS
    2.1. Genomics / 40
    2.2. Bioinformatics and Computational Biology / 57
    References / 58
    Further Reading / 59
    CHAPTER 3 METHODOLOGY FOR SEPARATION AND IDENTIFICATION OF PROTEINS AND THEIR INTERACTIONS
    3.1. Separation of Proteins Via a Multidimensional Approach / 61
    3.2. Determination of the Primary Structure of Proteins / 70
    3.3. Determination of the 3D Structure of a Protein / 83
    3.4. Determination of the Amount of Proteins / 86
    3.5. Structural and Functional Proteomics / 89
    References / 99
    Further Reading / 101
    CHAPTER 4 PROTEOMICS OF PROTEIN MODIFICATIONS
    4.1. Phosphorylation and Phosphoproteomics / 104
    4.2. Glycosylation and Glycoproteomics / 107
    4.3. Ubiquitination and Ubiquitinomics / 110
    4.4. Miscellaneous Modifications of Proteins / 112
    References / 113
    Further Reading / 113
    CHAPTER 5 PROTEOMICS OF PROTEIN-PROTEIN INTERACTIONS/INTERACTOMES
    5.1. Protein--Protein Interactions (PPI) in Vivo / 116
    5.2. Analysis of Protein Interactions in Vitro / 118
    5.3. Analysis of Protein Interactions in Silico / 124
    5.4. Synthetic Genetic Methods to Determine Protein Interactions / 125
    5.5. Interactomes / 125
    5.6. Evolution and Conservation of Interactomes / 132
    5.7. Interactomes and the Complexity of Organisms: It is the Number of Interactomes that Matters in Understanding the Complexity of an Organism and not the Number of Genes / 133
    5.8. Interaction of Proteins with Small Molecules / 133
    References / 134
    Further Reading / 134
    CHAPTER 6 APPLICATIONS OF PROTEOMICS I: PROTEOMICS， HUMAN DISEASE， AND MEDICINE
    6.1. Diseasome / 139
    6.2. Medical Proteomics / 139
    6.3. Clinical Proteomics / 148
    6.4. Metaproteomics and Human Health / 153
    6.5. Proteomics in Biotechnology and Industry of Drug Production / 154
    6.6. Metaproteomics of Microbial Fermentation / 155
    6.7. Beef Industry / 158
    6.8. Bioterrorism and Biodefense / 158
    References / 159
    Further Reading / 160
    CHAPTER 7 PROTEOMICS--FUTURE DEVELOPMENTS 163
    7.1. Technical Scope of Proteomics--Beyond Protein Identification / 163
    7.2. Scientific Scope of Proteomics--Control of Epigenesis / 165
    7.3. Medical Scope of Proteomics / 166
    7.4. Proteomics， Energy Production， and Bioremediation / 169
    7.5. Proteomics and Biodefense / 170
    References / 170
    Further Reading / 170
    INDEX