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Tissue-specific vascular endothelial signals and vector targeting. part B / edited by Renata Pasqualini. — San Diego : Academic Press, c2010. – (58.14/A244/v.69) |
Contents
Contents
Contributors ix
1. MR Molecular Imaging of Tumor Vasculature and Vascular Targets 1
I. Introduction 2
II. Structural， Functional， and Molecular Characteristics of Tumor Vasculature
III. Basis of Contrast in MR images 7
IV. Imaging Receptor Expression 16
V. Imaging Vascular Targeting 17
VI. Multimodal Molecular-Functional Imaging of Tumor Vasculature 19
VII. Conclusion 21
References 22
2. An Integrated Approach for the Rational Design of Nanovectors for Biomedical Imaging and Therapy 31
I. Introduction 32
II. Three Generations of Nanovectors 34
III. Mathematical Models for Predicting the Nanovectors Behavior 39
IV. Biophysical Characterization of Nanovectors 48
V. Vascular Targeting Moieties 50
VI. In vitro Assays for Nanovectors' Characterization 53
VII. Intravital Video Microscopy 55
VIII. Conclusions 57
References 59
3. Targeted Systemic Gene Therapy and Molecular Imaging of cancer: Contribution of the Vascular-Targeted AAVP Vector 65
I. Introduction 66
II. Targeted Gene Delivery 68
III. Conclusions and Perspectives 79
References 80
4. Cationic and Tissue-Specific Protein Transduction Domains: Identification， Characterization， and Therapeutic Application 83
I. Introduction 84
II. Cationic Protein Transduction Domains 84
III. Biopanning using Peptide Phage Display Libraries
IV. Peptide Phage Display Biopanning in Cell Culture for Transduction Peptides
V. Methods for Analysis of Transduction Efficiency
VI. Peptide Phage Display Biopanning in vivo for Transduction Peptides 91
VII. PTDs as Therapeutics 92
VIII. Conclusions 93
References 93
5. GRP78 Signaling Hub: A Receptor for Targeted Tumor Therapy 97
I. Introduction 99
II. GRP78 Association with Activated α2-Macroglobulin Promotes Cell Proliferation
III. GRP78 Autoantibody Predicts Poor Prognosis in Tumors but Shows Therapeutic Effects in Rheumatoid Arthritis 102
IV. GRP78 Promotes Endothelial Cell Survival in Association with T-Cadherin 103
V. Cripto Mitogenic Signaling is GRP78-Dependent 103
VI. Interaction of GRP78 with Plasminogen Kringle 5 and Microplasminogen Leads to Apoptosis or the UPR 104
VII. GRP78 Association with Extracellular Par-4 Activates the Extrinsic Apoptotic Pathway 105
VIII. GRP78 Inhibits Tissue Factor-Mediated Procoagulant Activity 107
IX. GRP78 Targeting Peptides Inhibit Tumor Growth 108
X. Conclusions 109
References 110
6 On the Synergistic Effects of Ligand-Mediated and Phage-Intrinsic Properties During In Vivo Selection 115
I. Introduction 116
II. Ligand-Receptor Mediated Effects 118
III. Synergistic Effects Intrinsic to Phage 124
IV. Conclusion 130
References 130
7 Strategies for Targeting Tumors and Tumor Vasculature for Cancer Therapy 135
I. Introduction 136
II. Isolated Limb Perfusion 136
III. Isolated Liver Perfusion 137
IV. Chemoembolization 138
V. Gene Therapy Strategies Available to Target the Tumor and the Tumor-Associated Vasculature 139
VI. Conclusions 148
References 148
Index 153
Color Plate Section at the end of the Book
