The PCR revolution : basic technologies and applications / edited by Stephen A. Bustin. — Cambridge ; New York : Cambridge University Press, 2010. – (58.17435/P783p)

Contents

    Contents
    
    Contributors
    Foreword by Russell Higuchi
    Preface
    I BASIC
    1 Real-time polymerase chain reaction
    2 Thermostable enzymes used in polymerase chain reaction
    3 Inventing molecular beacons
    4 Rapid polymerase chain reaction and melting analysis
    5 Polymerase chain reaction and fluorescence chemistries: deoxyribonucleic acid incarnate
    6 Analysis of microribonucleic acid expression by quantitative real-time polymerase chain reaction
    7 Miniaturized polymerase chain reaction for quantitative clinical diagnostics
    8 The road from qualitative to quantitative assay: What is next?
    9 Taking control of the polymerase chain reaction
    II APPLICATIONS
    10 Polymerase chain reaction-based methods for the detection of solid tumor cancer cells for clinical diagnostic and prognostic assays 155
    11 Polymerase chain reaction and infectious diseases
    12 Polymerase chain reaction and respiratory viruses
    13 Polymerase chain reaction and severe acute respiratory syndrome
    14 The MMR vaccine， measles virus， and autism - A cautionary tale
    15 Noninvasive prenatal diagnosis using cell-free fetal nucleic acids in maternal plasma
    16 Polymerase chain reaction-based analyses of nucleic acids from archival material
    17 Microarrays and quantitative real-time reverse transcriptase-polymerase chain reaction
    18 Polymerase chain reaction in the detection of genetic variation
    19 Polymerase chain reaction: A blessing and a curse for ancient deoxyribonucleic acid research
    Index
    Color Plates follow page 214