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G protein-coupled receptors : Essential methods / edited by David R. Poyner and Mark Wheatley. — Chichester, UK : John Wiley & Sons, 2010. – (58.17421/G111g) |
Contents
Contents
Preface
Contributors
1 Measurement of Ligand-G Protein-coupled Receptor Interactions
1.1 Introduction
1.2 Methods and approaches
References
2 Second Messenger Assays for G Protein-coupled Receptors: cAMP， Ca2+， Inositol Phosphates， ERK1/2
2.1 Introduction
2.2 Methods and approaches
2.3 Troubleshooting
References
3 Use of the [35S]GTPγS Binding Assay to Determine Ligand Efficacy at G Protein-coupled Receptors
3.1 Introduction
3.2 Methods and approaches
3.3 Troubleshooting
Acknowledgements
References
4 Quantitative Imaging of Receptor Trafficking
4.1 Introduction
4.2 Methods and approaches
4.3 Troubleshooting
References
5 Production of Recombinant G Protein-coupled Receptor in Yeast for Structural and Functional Analysis
5.1 Introduction
5.2 Methods and approaches
5.3 Troubleshooting
References
6 Monitoring GPCR-Protein Complexes Using Bioluminescence Resonance Energy Transfer
6.1 Introduction
6.2 Methods and approaches
6.3 Troubleshooting
References
7 Using Intramolecular Fluorescence Resonance Energy Transfer to Study Receptor Conformation
7.1 Introduction
7.2 Methods and approaches
7.3 Troubleshooting
References
8 A Disulfide Cross-linking Strategy Useful for Studying Ligand-induced Structural Changes in GPCRs
8.1 Introduction
8.2 Methods and approaches
8.3 General considerations， caveats and troubleshooting
Acknowledgements
References
9 Use of Fluorescence Correlation Spectroscopy to Study the Diffusion of G Protein-coupled Receptors
9.1 Introduction
9.2 Methods and approaches
9.3 Troubleshooting
References
10 Identification and Analysis of GPCR Phosphorylation
10.1 Introduction
10.2 Methods
Acknowledgements
References
11 Measurement and Visualization of G Protein-coupled Receptor Trafficking by Enzyme-linked Immunosorbent Assay and Immunofluorescence
11.1 Introduction
11.2 Methods and approaches
11.3 Troubleshooting
References
12 Substituted Cysteine Accessibility Method (SCAM)
12.1 Introduction
12.2 Methods and approaches
12.3 Troubleshooting
References
13 Homology Modelling of G Protein-coupled Receptors
13.1 Introduction
13.2 Methods and approaches
13.3 Troubleshooting
13.4 Automated methods for generating models of GPCRs
References
Appendix Site-directed Mutagenesis and Chimeras
A.1 Introduction
A.2 Why mutagenesis?
A.3 Troubleshooting
A.4 Conclusion
References
Index
