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Yeast : Molecular and cell biology / Horst Feldmann. — Weinheim : Wiley-VCH, c2010. – (58.88421/F312) |
Contents
Contents
Preface
Foreword
1 Introduction 1
1.1 Historical Aspects 1
1.2 Yeast as a Eukaryotic Model System
2 The "Early Days" of Nucleic Acids 5
2.1 Molecular Biology of tRNA 5
2.2 Ribosomes， Ribosomal RNAs， and Ribosomal Proteins
2.3 Messenger RNAs 10
3 Genetic Engineering and Reverse Genetics 15
3.1 The Molecular Revolution 15
3.2 Transformation of Yeast Cells 16
3.3 More Genetic Tools from Yeast Cells 26
3.4 The Two-Hybrid System 17
4 Cell Structures and Functions 19
4.1 The Yeast Cell 19
4.2 Yeast Envelope and Cell Skeleton 19
4.3 Yeast Chromosome Structure and Function 21
5 Cellular Dynamics 33
5.1 Cell Propagation 33
5.2 Regulatory ATPases - AAA+ Proteins 36
5.3 Protein Modification by Proteins and Programmed Protein Degradation 40
5.4 The Yeast Cell Cycle， Cell Division， and Chromosome Segregation The Cell Cycle
5.5 Intracellular Protein Sorting and Transport 58
5.6 Membrane Transporters in Yeast 75
6 Anatomy of the Transcription Machinery and Auxiliary Complexes: Chromatin Remodeling 85
6.1 Transcriptional Regulation and Transcription Factors 85
6.2 RNA Polymerases and Cofactors 86
6.3 Coupling Transcription to Pre-mRNA Processing 99
6.4 DNA Repair Connected to Transcription 101
6.5 Modification of Chromatin During Transcription 102
7 Molecular Signaling Cascades and Gene Regulation 109
7.1 MAP Kinase Pathways 109
7.2 General Control by Gene Repression 115
7.3 Gene Regulation by Nutrients 117
7.4 Stress Responses in Yeast 129
7.5 Posttranscriptional Control of Gene Expression in Yeast 139
7.6 Protein Splicing - Yeast Inteins 143
8 Yeast Organellar Biogenesis and Function 147
8.1 Mitochondria 147
8.2 Peroxisomes 158
9 Genome， Proteome， Transcriptome， Metabolome， and Regulatory Networks 165
9.1 The Yeast Genome Sequencing Project 165
9.2 Characteristics of the Yeast Genome 166
9.3 Functional Genomics 171
9.4 Hemiascomycetous Yeasts and Evolutionary Aspects
10 Disease Genes in Yeast 185
10.1 General Aspects 185
10.2 Trinucleotide Repeats and Neurodegenerative Diseases 187
10.3 Aging and Age-Related Disorders 190
10.4 Mitochondrial Diseases 191
11 Yeast in Biotechnology 197
11.1 Fermentation and Metabolic Engineering 197
11.2 Biopharmaceuticals from Health-Care Industries 201
11.3Biomedical Research 204
11.4 Environmental Technologies: Cell-Surface Display 204
12 Epilogue: The Future of Yeast Research 207
References 209
Appendix: Online Resources Useful in Yeast Research 327
Index 329
