Cyclooxygenases : methods and protocols / edited by Samir S. Ayoub, Roderick J. Flower, Michael P. Seed. — [Totowa, N.J.] ; London : Humana, c2010. – (58.17/M592/v.644) |
Contents
Contents
Preface
Contributors
PART I IN VITRO PROTOCOLS FOR STUDYING EXPRESSION AND ACTIVITY OF CYCLOOXYGENASE ENZYMES AND CHABACTERISATION OF THEIR INHIBITION PATTERNS
1. In Vitro Cyclo-oxygenase Expression and Activity Protocols Introduction to Part I
2. Techniques Used to Study Regulation of Cyclooxygenase-2 Promoter Sites 11
3. Purification of Recombinant Human COX-1 and COX-2
4. Cloning and Expression of Cyclooxygenase- 1 and Cyclooxygenase-2
5. Expression of Cyclooxygenase Isoforms in tile Baculovirus Expression System 45
6. Peroxidase Active Site Activity Assay
7. Study of Inhibitors of the PGH Synthases for which Potency Is Regulated by the Redox State of the Enzymes
8. Different Methods for Testing Potential Cyclooxygenase-1 and Cyclooxygenase-2 Inhibitors
9. In Vitro Cyclooxygenase Activity Assay in Tissue Homogenates
10. Techniques Used to Characterize the Binding of Cyclooxygenase Inhibitors to the Cyclooxygenase Active Site
11. In Vitro Assays for Cyclooxygenase Activity and Inhibitor Characterization
PART II EXTRACTION AND MEASUREMENT OF PROSTANOIDS AND ISOPROSTANES
12. Extraction and Measurement of Prostanoids and Isoprostanes Introduction to Part II
13. Prostanoid Extraction and Measurement
14. Measurement of 8-Iso-Prostaglandin F2α in Biological Fluids as a Measure of Lipid Peroxidation
PART III IN Vivo MODELS TO STUDY INVOLVEMENT OF CYCLOOXYGENASE PRODUCTS IN HEALTH AND DISEASE
15. In Vivo Models to Study Cyclooxygenase Products in Health and Disease: Introduction to Part III 181
16. Protocols to Assess the Gastrointestinal Side Effects Resulting from Inhibition of Cyclo-Oxygenase Isoforms 189
17. Cyclooxygenase Enzymes and Their Products in the Carrageenan-Induced Pleurisy in Rats 201
18. Iloprost-Induced Nociception: Determination of the Site of Anti-nociceptive Action of Cyclooxygenase Inhibitors and the Involvement of Cyclooxygenase Products in Central Mechanisms of Nociception 207
Index