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Hepatitis C : methods and protocols / edited by Hengli Tang. — 2nd ed. — New York, N.Y. : Humana ; [London : Springer, distributor], c2009. – (58.17/M592/v.510)

Contents

    Contents
    
    PART I. DETECTION AND QUANTIFICATION OF HCV
    1 Detection and Quantification of Serum or Plasma HCV RNA: Mini Review of Commercially Available Assays
    2 Simultaneous Detection of Anti-HCV Antibody and HCV Core Antigen
    3 Immunohistochemical Detection of HCV Proteins in Liver Tissue
    PART II. HCV NOMENCLATURE, GENOTYPING, AND QUASISPECIES
    4 Nomenclature and Numbering of the Hepatitis C Virus
    5 A Real-Time Taqman Method for Hepatitis C Virus Genotyping and Methods for Further Subtyping of Isolates
    6 Characterization of HCV Quasispecies in the Hypervariable Region 1 (HVR 1) by In Vitro Translation and Mass Spectrometry
    PART III. HCV REPLICATION IN VITRO
    7 Purification and crystallization of NS5A domain I of hepatitis C virus
    8 NS5A Phosphorylation and Hyperphosphorylation
    9 Preparation and Handling of Hepatitis C Viral Proteins NS3 and NSSB for Structural Studies
    10 Structural and Functional Analysis of the HCV p7 Protein
    11 HCV Replicons: Overview and Basic Protocols
    12 Reverse Transcription PCR-Based Sequence Analysis of Hepatitis C Virus Replicon RNA
    13 Studying HCV RNA Synthesis In Vitro with Replication Complexes
    14 Proteomics Study of the Hepatitis C Virus Replication Complex
    15 Investigation of the Hepatitis C Virus Replication Complex
    16 Regulation of Interferon Regulatory Factor 3-Dependent Innate Immunity by the HCV NS3/4A Protease
    17 Selection and Characterization of Drug-Resistant HCV Replicons In Vitro with a Flow Cytometry-Based Assay
    18 High-Throughput Screening of HCV RNA Replication Inhibitors by Means of a Reporter Replicon System
    19 Inhibition of HCV Replication by Small Interfering RNA
    PART IV. HCV ENTRY AND INFECTION
    20 Cellular Receptors and HCV Entry
    21 Studying HCV Cell Entry with HCV Pseudoparticles (HCVpp)
    22 Screening of Small-Molecule Compounds as Inhibitors of HCV Entry
    23 Isolation of JFH-1 Strain and Development of an HCV Infection System
    24 Measuring HCV Infectivity Produced in Cell Culture and In Vivo
    25 Genotype la HCV (H77S) Infection System
    26 Full-Length Infectious HCV Chimeras
    27 Adaptation of the Hepatitis C Virus to Cell Culture
    28 Primary Human Hepatocyte Culture for HCV Study
    29 In Vivo Study of HCV in Mice with Chimeric Human Livers
    PART V. IMMUNITY AND MODELING
    30 Determination of HCV-Specific T-Cell Activity
    31 Analysis of HCV-Specific T Cells by Flow Cytometry
    32 Detection of Neutralizing Antibodies with HCV Pseudoparticles (HCVpp)
    33 Mathematical Modeling of HCV Infection and Treatment
    Index 455