Hepatitis C : methods and protocols / edited by Hengli Tang. — 2nd ed. — New York, N.Y. : Humana ; [London : Springer, distributor], c2009. – (58.17/M592/v.510) |
Contents
Contents
PART I. DETECTION AND QUANTIFICATION OF HCV
1 Detection and Quantification of Serum or Plasma HCV RNA: Mini Review of Commercially Available Assays
2 Simultaneous Detection of Anti-HCV Antibody and HCV Core Antigen
3 Immunohistochemical Detection of HCV Proteins in Liver Tissue
PART II. HCV NOMENCLATURE, GENOTYPING, AND QUASISPECIES
4 Nomenclature and Numbering of the Hepatitis C Virus
5 A Real-Time Taqman Method for Hepatitis C Virus Genotyping and Methods for Further Subtyping of Isolates
6 Characterization of HCV Quasispecies in the Hypervariable Region 1 (HVR 1) by In Vitro Translation and Mass Spectrometry
PART III. HCV REPLICATION IN VITRO
7 Purification and crystallization of NS5A domain I of hepatitis C virus
8 NS5A Phosphorylation and Hyperphosphorylation
9 Preparation and Handling of Hepatitis C Viral Proteins NS3 and NSSB for Structural Studies
10 Structural and Functional Analysis of the HCV p7 Protein
11 HCV Replicons: Overview and Basic Protocols
12 Reverse Transcription PCR-Based Sequence Analysis of Hepatitis C Virus Replicon RNA
13 Studying HCV RNA Synthesis In Vitro with Replication Complexes
14 Proteomics Study of the Hepatitis C Virus Replication Complex
15 Investigation of the Hepatitis C Virus Replication Complex
16 Regulation of Interferon Regulatory Factor 3-Dependent Innate Immunity by the HCV NS3/4A Protease
17 Selection and Characterization of Drug-Resistant HCV Replicons In Vitro with a Flow Cytometry-Based Assay
18 High-Throughput Screening of HCV RNA Replication Inhibitors by Means of a Reporter Replicon System
19 Inhibition of HCV Replication by Small Interfering RNA
PART IV. HCV ENTRY AND INFECTION
20 Cellular Receptors and HCV Entry
21 Studying HCV Cell Entry with HCV Pseudoparticles (HCVpp)
22 Screening of Small-Molecule Compounds as Inhibitors of HCV Entry
23 Isolation of JFH-1 Strain and Development of an HCV Infection System
24 Measuring HCV Infectivity Produced in Cell Culture and In Vivo
25 Genotype la HCV (H77S) Infection System
26 Full-Length Infectious HCV Chimeras
27 Adaptation of the Hepatitis C Virus to Cell Culture
28 Primary Human Hepatocyte Culture for HCV Study
29 In Vivo Study of HCV in Mice with Chimeric Human Livers
PART V. IMMUNITY AND MODELING
30 Determination of HCV-Specific T-Cell Activity
31 Analysis of HCV-Specific T Cells by Flow Cytometry
32 Detection of Neutralizing Antibodies with HCV Pseudoparticles (HCVpp)
33 Mathematical Modeling of HCV Infection and Treatment
Index 455