Protein secretion : methods and protocols / edited by Anastassios Economou. — New York : Springer, 2010. – (58.17/M592/v.619)

Contents

    Contents
    
    Contributors
    1. The Extraordinary Diversity of Bacterial Protein Secretion Mechanisms
    2. In Vitro and In Vivo Approaches to Studying the Bacterial Signal Peptide Processing
    3. Membrane Insertion of Small Proteins
    4. Membrane Protein Insertion in E. coli
    5. Study of Polytopic Membrane Protein Topological Organization as a Function of Membrane Lipid Composition
    6. In Vivo Analysis of Protein Translocation to the Escherichia coli Periplasm 103
    7. Sorting of Bacterial Lipoproteins to the Outer Membrane by the Lo1 System
    8. Purification and Functional Reconstitution of the Bacterial Protein Translocation Pore， the SecYEG Complex
    9. Reconstitution of the SecY Translocon in Nanodiscs
    10. In Vitro Assays to Analyze Translocation of the Model Secretory Preprotein Alkaline Phosphatase
    11. Characterization of Interactions Between Proteins Using Site-Directed Spin Labeling and Electron Paramagnetic Resonance Spectroscopy..
    12. Analysis of Tat Targeting Function and Twin-Arginine Signal Peptide Activity in Escherichia coli
    13. Site-Specific Cross-Linking of In Vitro Synthesized E. coli Preproteins for Investigating Transmembrane Translocation Pathways 217
    14. Tracking the Secretion of Fluorescently Labeled Type III Effectors from Single Bacteria in Real Time 241
    15. Comparative Analysis of Cytoplasmic Membrane Proteomes of Escherichia coli Using 2D Blue Native/SDS-PAGE
    16. Using Hidden Markov Models to Discover New Protein Transport Machines 271
    17. Bioinformatics Predictions of Localization and Targeting 285
    18. The Chloroplast Protein Import Machinery: A Review
    19. Measurement of the Energetics of Protein Transport Across the Chloroplast Thylakoid Membrane
    20. In Vitro Dissection of Protein Translocation into the Mammalian Endoplasmic Reticulum
    21. In Vitro Reconstitution of the Selection， Ubiquitination， and Membrane Extraction of a Polytopic ERAD Substrate
    22. Studying the ArfGAP-Dependent Conformational Changes in SNAREs
    23. Studying Endoplasmic Reticulum Function In Vitro Using siRNA 389
    24. High-Quality Immunofluorescence of Cultured Cells
    25. Trapping Oxidative Folding Intermediates During Translocation to the Intermembrane Space of Mitochondria: In Vivo and In Vitro Studies 411
    26. Native Techniques for Analysis of Mitochondrial Protein Import 425
    Subject Index