Mouse cell culture : methods and protocols / edited by Andrew Ward and David Tosh. — New York ; London : Humana, c2010. – (58.17/M592/v.633)

Contents

    Contents
    
    Preface
    Contributors
    1. The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies
    2. Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures
    3. Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells 29
    4. The Embryonic Kidney: Isolation， Organ Culture， immunostaining and RNA Interference
    5. Explant Culture of Mouse Embryonic Whole Lung， Isolated Epithelium， or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular Differentiation
    6. Isolation， Culture， and Characterisation of Mouse Embryonic oesophagus and Intestine
    7. Isolation and Culture of Embryonic Pancreas and Liver
    8. Isolation and Culture of Mouse Satellite Cells
    9. Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte Cultures
    10. Short- and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes
    11. Isolation， Culture and Analysis of Mouse Mammary Epithelial Cells
    12. Isolation and Culture of Mouse Pancreatic Islets for Ex Vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes
    13. Isolation and Culture of Adult Mouse Hepatocytes 185
    14. Isolation and Culture of Mouse Intestinal Cells
    15. Derivation of Primary Choroid Plexus Epithelial Cells from the Mouse 207
    16. The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluorescent Cytochemistry 221
    17. Cell Culture of Primary Cerebellar Granule Cells 233
    18. Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain
    Subject Index