Plant stress tolerance : methods and protocols / edited by Ramanjulu Sunkar. — [Totowa, N.J.] : Humana Press, c2010. – (58.17/M592/v.639)

Contents

    Contents
    
    Preface
    Contributors
    PART I REVIEWS AND OVERVIEW CHAPTERS
    1. Dehydration Tolerance in Plants
    2. Approaches to Identifying Genes for Salinity Tolerance and the importance of Timescale
    3. Gene Regulation During Cold Stress Acclimation in Plants 39
    4. Redox-Dependent Regulation， Redox Control and Oxidative Damage in Plant Cells Subjected to Abiotic Stress
    5. Array Platforms and Bioinformatics Tools for the Analysis of Plant Transcriptome in Response to Abiotic Stress
    6. Metabolic Engineering of Glyoxalase Pathway for Enhancing Stress Tolerance in Plants 95
    PART II GENOME-WIDE APPROACHES FOR IDENTIFICATION OF STRESS-REGULATED GENES， PROTEINS， AND SMALL RNAs
    7. Genetic Screens to Identify Plant Stress Genes 121
    8. Arabidopsis Tiling Array Analysis to Identify the Stress-Responsive Genes 141
    9. Identification of Stress-Responsive Genes in Plants Using Suppression Subtraction Hybridization: Ozone Stress as an Example
    10. Identification of DNA-Binding Proteins and Protein-Protein Interactions by Yeast One-Hybrid and Yeast Two-Hybrid Screen 171
    ll. Functional Characterization of Water-Deficit Stress Responsive Genes Using RNAi 193
    12. Difference Gel Electrophoresis as a tool to Discover Stress-Regulated Proteins
    13. Thiol-Disulfide Redox Proteomics in Plant Research 219
    14. Cloning of Stress-Responsive MicroRNAs and other Small RNAs from Plants
    15. An Array Platform for Identification of Stress-Responsive MicroRNAs in Plants
    PART III ANTIOX1DANT ENZYMES AND METABOLITES
    16. Spectrophotometric Assays for Antioxidant Enzymes in Plants 273
    17. Affinity Purification and Determination of Enzymatic Activity of Recombinantly Expressed Aldehyde Dehydrogenases 281
    18. Determination and Detection of Reactive Oxygen Species (ROS)， Lipid Peroxidation， and Electrolyte Leakage ill Plants 291
    PART IV OSMOTIC ADJUSTMENT AND ION MEASUREMENTS
    19. Quantification of Water Stress-Induced Osmotic Adjustment and Proline Accumulation for Arabidopsis thaliana Molecular Genetic Studies 301
    20. Methods for Determination of Proline in Plants 317
    21. A New Method for Accurately Measuring A1-Pyrroline-5-Carboxylate Synthetase Activity 333
    22. Extraction and Analysis of Soluble Carbohydrates 341
    23. Measuring Soluble Ion Concentrations (Na+， K+， Cl-) in Salt-Treated Plants
    Subject Index 383