Hepatocytes : methods and protocols / edited by Patrick Maurel. — Totowa, N.J. : Humana ; London : Springer [distributor], c2010. – (58.17/M592/v.640) |
Contents
Contents
Foreword
Preface
Contributors
1. General Review on In Vitro Hepatocyte Models and Their Applications
2. Human Foetal Hepatocytes: Isolation, Characterization, and Transplantation 41
3. Isolation and Culture of Primary Hepatocytes from Resected Human Liver Tissue
4. Optimisation of the Cryopreservation of Primary Hepatocytes 83
5. Cryopreservation of Human Hepatocytes for Clinical Use 107
6. Hepatocyte Differentiation 115
7. Reversible Manipulation of Apoptosis Sensitivity in Cultured Hepatocytes by Matrix-Mediated Manipulation of Signaling Activities 139
8. Markers and Signaling Factors for Stem Cell Differentiation to Hepatocytes: Lessons from Developmental Studies 157
9. Hepatic Stem Cells
10. Hepatic Stem Cells and Liver Development 181
11. Generation of Hepatocytes from Human Embryonic Stem Cells 237
12. Isolation and Culture of Adult Human Liver Progenitor Cells: In Vitro Differentiation to Hepatocyte-Like Cells 247
13. The HepaRG Cell Line: Biological Properties and Relevance as a Tool for Cell Biology, Drug Metabolism, and Virology Studies 261
14. Transdifferentiation of Pancreatic Cells to Hepatocytes 273
15. Evaluation of Drug Metabolism, Drug-Drug Interactions, and In Vitro Hepatotoxicity with Cryopreserved Human Hepatocytes 281
16. The Use of Human Hepatocytes to Investigate Drug Metabolism and CYP Enzyme Induction 295
17. The Use of Hepatocytes to Investigate UDP-Glucuronosyltransferases and Sulfotransferases
18. The Use of Hepatocytes to Investigate Drug Uptake Transporters 327
19. Metabonomic Studies on Human Hepatocyte in Primary Culture 355
20. The Application of HepRG Cells in Evaluation of Cytochrome P450 Induction Properties of Drug Compounds 375
21. The Use of Hepatocytes to Investigate Drug Toxicity
22. The Use of Human Hepatocytes to Investigate Bile Acid Synthesis 417
23. Use of Human Hepatocytes to Investigate Blood Coagulation Factor 431
24. Use of Human Hepatocytes to Investigate HCV Infection 447
25. The Use of Hepatocytes to Investigate HDV Infection: The HDV/HepaRG Model 463
26. Rodent Models of Liver Repopulation
27. Chimeric Mice with Humanized Liver: Tools for tile Study of Drug Metabolism, Excretion, and Toxicity 491
28. Bioartificial Liver Support Systems
29. Human Hepatocyte Transplantation 525
Subject Index 535