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Plant secondary metabolism engineering : methods and applications / edited by Arthur Germano Fett-Neto. — Totowa, N.J. : Humana, 2010. – (58.17/M592/v.643) |
Contents
Contents
Contributors
1. Plant Secondary Metabolism and Challenges in Modifying Its Operation: An Overview
2. Suppression Subtractive Hybridization as a Tool to Identify Anthocyanin Metabolism-Related Genes in Apple Skin
3. Identification of Regulatory Protein Genes Involved in Alkaloid Biosynthesis Using a Transient RNAi System 33
4. Site-Directed Mutagenesis and Saturation Mutagenesis for the Functional Study of Transcription Factors Involved in Plant Secondary Metabolite Biosynthesis
5. Isolation of Proteins Binding to Promoter Elements of Alkaloid Metabolism-Related Genes Using Yeast One-Hybrid 59
6. Modulation of Carotenoid Accumulation in Transgenic Potato by Inducing Chromoplast Formation with Enhanced Sink Strength 77
7. Over-expression of Rate-Limiting Enzymes to Improve Alkaloid Productivity 95
8. Microbial Expression of Alkaloid Biosynthetic Enzymes for Characterization of Their Properties
9. Producing a Recombinant Flavin-Containing Monooxygenase from Coffea arabica in Escherichia coli for Screening of Potential Natural Substrates 121
10. Efficient Production of Active Form Recombinant Cassava Hydroxynitrile Lyase Using Escherichia coli in Low-Temperature Culture 133
11. Introduction of the Early Pathway to Taxol Biosynthesis in Yeast by Means of Biosynthetic Gene Cluster Construction Using SOE-PCR and Homologous Recombination 145
12. Biocatalytic Synthesis of Tritium (3H)-Labelled Taxa-4(5)， 11(12)-diene， the Pathway Committing Precursor of the Taxoid Diterpenoids 165
13. USER Cloning and USER Fusion: The Ideal Cloning Techniques for Small and Big Laboratories 185
14. Enrichment of Carotenoids in Flaxseed by Introducing a Bacterial Phytoene Synthase Gene 201
15. Metabolic Engineering by Plastid Transformation as a Strategy to Modulate Isoprenoid Yield in Plants 213
16. Engineering High Yields of Secondary Metabolites in Rubia Cell Cultures Through Transformation with Rol Genes 229
17. Flow Cytometric Methods to Investigate Culture Heterogeneities for Plant Metabolic Engineering 243
18. Phenylpropanoid Biosynthesis in Leaves and Glandular Trichomes of Basil (Ocimum basilicum L.)
19. Fusion with Fluorescent Proteins for Subcellular Localization of Enzymes Involved in Plant Alkaloid Biosynthesis 275
20. Immunohistochemical Localisation of a Putative Flavonoid Transporter in Grape Berries 291
21. Electrogenic Bromosulfalein Transport in Isolated Membrane Vesicles: Implementation in Both Animal and Plant Preparations for the Study of Flavonoid Transporters 307
Subject Index 337
