Natural killer cell protocols : cellular and molecular methods / edited by Kerry S. Campbell. — 2nd ed. — New York : Humana, c2010. – (58.17/M592/v.612) |
Contents
Contents
Preface
Contributors
1. Purification of Human NK Cell Developmental Intermediates from Lymph Nodes and Tonsils
2. In Vitro Development of Human Killer-Immunoglobulin Receptor-Positive NK Cells
3. Subset Analysis of Human and Mouse Mature NK Cells 27
4. Assessing Licensing of N K Cells 39
5. Use of Stem Cell Radiation Chimeras to Analyze How Domains of Specific Proteins Impact on Murine NK Cell Development In Vivo
6. Use of Transfected Drosophila $2 Cells to Study NK Cell Activation 67
7. Natural Killer Cell Conjugate Assay Using Two-Color Flow Cytometry 89
8. Studying NK Cell/Dendritic Cell Interactions 97
9. Analysis of the NK Cell Immunological Synapse 127
10. Measuring Intracellular Calcium Signaling in Murine NK Cells by Flow Cytometry 149
11. Intracellular Staining for Analysis of the Expression and Phosphorylation of Signal Transducers and Activators of Transcription (STATs) in NK Cells 159
12. A Model System for Studying NK Cell Receptor Signaling 177
13. Expression of cDNAs in Human Natural Killer Cell Lines by Retroviral Transduction 199
14. Lentiviral Gene Transduction in Human and Mouse NK Cell Lines
15. Introduction of shRNAs into Human NK-Like Cell Lines with Retrovirus
16. Introduction of shRNAs into Primary NK Cells with Lentivirus 233
17. Methods to Identify and Characterize Different NK Cell Receptors and Their Ligands 249
18. Generating NK Cell Receptor-Fc Chimera Proteins from 293T Cells and Considerations of Appropriate Glycosylation 275
19. Identification of NK Cell Receptor Ligands Using a Signaling Reporter System 285
20. Determining Ligand Specificity of Ly49 Receptors 299
21. Probing the Interactions of NK Cell Receptors with Ligand Expressed in trans and cis 313
22. A Simple Method to Measure NK Cell Cytotoxicity In vivo 325
23. Functional Analysis of Human NK Cells by Flow Cytometry 335
24. Analysis of the KIR Repertoire in Human NK Cells by Flow Cytometry 353
25. KIR Genotyping by Multiplex PCR-SSP 365
26. Identification and Analysis of Novel Transcripts and Promoters in the Human Killer Cell Immunoglobulin-like Receptor (KIR) Genes 377
27. Use of Inbred Mouse Strains to Map Recognition Receptors of MCMV Infected Cells in the NK Cell Gene Locus 393
28. Studying NK Cell Responses to Ectromelia Virus Infections in Mice 411
29. Activation of Human NK Cells by Malaria-Infected Red Blood Cells
30. Natural Killer Cells in Human Pregnancy
31. Analysis of Uterine Natural Killer Cells in Mice
32. Isolation of NK Cells and NK-Like Cells from the Intestinal Lamina Propria
Appendix
Index