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Live cell imaging : a laboratory manual / edited by Robert D. Goldman, Jason R. Swedlow, David L. Spector. — 2nd ed. — Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2010. – (58.15/L722/2nd ed.) |
Contents
Contents
Preface， xv
SECTION 1 DETECTION AND APPROACHES TO LIVE CELL IMAGING， 1
1 Fluorescent Protein Tracking and Detection， 3
2 Constructing and Expressing Fluorescent Protein Fusions， 35
3 Micropatterning Cell-Substrate Adhesions Using Linear Polyacrylamide as the Blocking Agent， 43
4 CCD Cameras for Fluorescence Imaging of Living Cells， 53
5 Fluorescence Perturbation Techniques to Study Mobility and Molecular Dynamics of Proteins in Live Cells: FRAP， Photoactivation， Photoconversion， and FLIP， 67
6 Imaging Protein States in Cells， 95
7 A Versatile， Multicolor Total Internal Reflection Fluorescence and Spinning-Disk Confocal Microscope System for High-Resolution Live Cell Imaging， 119
8 Confocal Microscopy， Deconvolution， and Structured Illumination Methods， 139
9 Atomic-Force Microscopy for Biological Imaging and Mechanical Testing across Length Scales， 183
10 OMX: A New Platform for Multimodal， Multichannel Wide-Field Imaging， 203
11 Digital Scanned Laser Light Sheet Fluorescence Microscopy， 215
12 First Steps for Fluorescence Correlation Spectroscopy of Living Cells， 229
13 Tracking and Quantitative Analysis of Dynamic Movements of Cells and Particles， 239
14 Imaging Techniques for Measuring the Materials Properties of Cells， 257
15 Computational Image Analysis of Cellular Dynamics: A Case Study Based on Particle Tracking， 271
16 Software Tools， Data Structures， and Interfaces for Microscope Imaging， 283
17 High-Throughput Microscopy Using Live Mammalian Cells， 297
SECTION 2 IMAGING OF LIVE CELLS AND ORGANISMS， 315
18 In Vivo Imaging of Mammalian Cells， 317
19 Live Cell Imaging of Yeast， 333
20 Live Imaging of Caenorhabditis elegans， 351
21 Live Cell Imaging of Plants， 371
22 Pushing the Limits of Live Cell Imaging in Drosophila， 387
23 Dynamic， Long-Term， In Vivo Imaging of Tumor-Stroma Interactions in Mouse Models of Breast Cancer Using Spinning-Disk Confocal Microscopy， 419
24 High-Resolution Multiphoton Imaging of Tumors In Vivo， 441
25 Correlated Live Cell Light and Electron Microscopy Using Tetracysteine Tags and Biarsenicals， 463
26 Intravital Microscopy of Normal and Diseased Tissues in the Mouse， 475
27 Imaging Lipids in Living Cells， 523
28 Development of Mammalian Cell Lines with lac Operator-Tagged Chromosomes， 541
29 Imaging Gene Expression in Living Cells， 565
30 Studying Mitosis in Cultured Mammalian Cells， 571
31 Imaging Intermediate Filament Proteins in Living Cells， 583
32 Methods for Expressing and Analyzing GFP-Tubulin and GFP-Microtubule- Associated Proteins， 605
33 Imaging of Membrane Systems and Membrane Traffic in Living Cells， 623
34 Imaging Live Cells under Mechanical Stress， 641
35 Imaging Single Molecules Using Total Internal Reflection Fluorescence Microscopy， 659
36 Cellular Imaging Using Total Internal Reflection Fluorescence Microscopy， 675
37 Visualization and Quantification of Single RNA Molecules in Living Cells， 697
Appendix: Cautions， 713
Index， 721
