 |
Liposomes. part G / edited by Nejat Duzgunes. — Amsterdam : Elsevier Academic Press, c2009. – (58.17435/C719/v.465) |
Contents
CONTENTS
Contributors
Preface
Volumes in Series
Section I. Advances in Liposome Formation and
Characterization
1. Spontaneously Formed Unilamellar Vesicles
1. Introduction
2. Preparation of Spontaneously Forming ULVs
3. Characterization of ULVs
4. ULV Stability
5. Parameters Affecting ULVs
6. Mechanism of ULV Formation
7. Encapsulation and Controlled Release Mechanism of Spontaneously Formed ULVs
8. Application
9. Concluding Remarks
Acknowledgement
References
2. Use of Acoustic Sensors to Probe the Mechanical Properties of Liposomes
1. Introduction
2. Acoustic Measurements
3. Experimental Procedures
4. Change in ΔPh and ΔA for Adsorbed Vesicles as a Function of Cholesterol Content
5. Comparison of the Results to Various Model Systems
6. Can the Response Be Explained in Terms of Variable Slip at the Surface?
7. Displacements Associated with the Liposomes
8. Concluding Remarks
Acknowledgments
References
3. Liposome Characterization by Quartz Crystal Microbalance Measurements and Atomic Force Microscopy
1. Introduction
2. Liposomes Assessed by AFM
3. QCM Measurements of Intact Liposomes
4. Surface-Bound Liposomes in Perspective and General Conclusions
References
4. Mixing Solutions in Inkjet Formed Vesicles
1. Introduction
2. Unilamellar Vesicle Formation by Microfluidic Encapsulation
3. Determination of Encapsulation Fraction within Vesicles
4. Concluding Remarks
References
5. Recombinant Proteoliposomes Prepared Using Baculovirus Expression Systems
1. Introduction
2. Principles of Recombinant Proteoliposome Preparation
3. Construction of Recombinant AcNPVs
4. Expression of Recombinant Proteins on BV Envelopes
5. Preparation of Proteoliposomes by Fusion of BVs with Liposomes
6. Concluding Remarks
References
6. Block Liposomes: Vesicles of Charged Lipids with Distinctly Shaped Nanoscale Sphere-， Pear-， Tube-， or Rod-Segments 111
1. Introduction
2. Liposome Preparation
3. Microscopy
4. Design and Synthesis of MVLBG2
5. Phase Behavior of MVLBG2/DOPC Lipid Mixtures
6. Concluding Remarks
Acknowledgments
References
7. Microfluidic Methods for Production of Liposomes
1. Introduction
2. Conventional Technologies for Production of Liposomes
3. Microfluidic Technologies for Synthesis of Nanoparticles
4. Microfluidic Technologies for Production of Liposomes
5. Concluding Remarks
Acknowledgments
References
8. Constructing Size Distributions of Liposomes from Single-Object Fluorescence Measurements
1. Introduction
2. How Particle Size can be Obtained from Intensity Distributions
3. Vesicle Preparation and Immobilization
4. Image Acquisition
5. Image Processing
6. Intensity and Size Distributions
7. Multilamellarity Assay
8. Measuring Membrane-Curvature Selective Protein Binding
9. Concluding Remarks
References
9. Giant Unilamellar Vesicle Electroformation: From Lipid Mixtures to Native Membranes Under Physiological Conditions 161
1. Introduction
2. General GUV Electroformation Protocol
3. Methods for GUV Electroformation from Lipid Mixtures， Liposomes， and Native Membranes
4. Concluding Remarks
References
Section II. Liposomes in Therapeutics
10. Liposomal Boron Delivery for Neutron Capture Therapy
1. Introduction
2. Boron-Encapsulation Approach
3. Boron Lipid-Liposome Approach
4. nido-Carborane Lipid Liposomes
5. Transferrin-Conjugated nido-Carborane Lipid Liposomes
6. closo-Dodecaborate Lipid Liposomes
7. Concluding Remarks
References
11. Production of Recombinant Proteoliposomes for Therapeutic Uses
1. Introduction
2. Expression of Bak Protein Using a Bacterial Cell-Free Expression System
3. Scale-Up Production of Bak Proteoliposomes
4. Bak Proteoliposome Production
5. Liposome Preparation
6. Proteoliposome Purification
7. Analysis of the Purified Bak Proteoliposomes
8. Transmission Electron Microscopy
9. Apoptosis Induction in Cancer Cell Lines: Caspase 9 Activation
10. Concluding Remarks
Acknowledgments
References
12. Liposome-Mediated Therapy of Neuroblastoma 225
1. Introduction
2. Materials
3. Untargeted Liposomes Entrapping Doxorubicin
4. Tumor-Targeted Liposomal Chemotherapy
5. Vascular-Targeted Liposomal Chemotherapy
6. Liposomes Entrapping Fenretinide (HPR)
7. Antisense Oligonucleotide-Entrapped Liposomes
8. Gold-Containing Liposomes
Acknowledgments
References
13. Tumor-Specific Liposomal Drug Release Mediated by Liposomase
1. Introduction
2. Tumor Models
3. Generation of C. novyi Spores and C. novyi-NT
4. Preparation of Liposomal Formulations
5. Combination Therapy with C. novyi-NT Spores and Liposomes
6. Purification and Identification of Liposomase
7. Conclusion and Future Perspectives
References
14. Targeted Lipoplexes for siRNA Delivery
1. Introduction
2. Liposome and Complex Preparation
3. Physicochemical Characterization of the Complexes
4. Assessment of siRNA Protection
5. Assessment of Lipoplex Internalization and Biological Activity In Vitro
6. Cell Viability Studies
7. Concluding Remarks
References
15. Mucosal Delivery of Liposome-Chitosan Nanoparticle Complexes
1. Introduction
2. Preparation of Liposome-Chitosan Nanoparticle (L/CS-NP) Complexes
3. Characterization of (L/CS-NP) Complexes
4. Results
5. Conclusions and Prospects
Acknowledgment
References
16. Antiangiogenic Photodynamic Therapy with Targeted Liposomes 313
1. Introduction 314
2. PDT with PEG-Coated Liposomal BPD-MA 316
3. PDT with Polycation-Coated Liposomal BPD-MA 318
4. PDT with Tumor Angiogenic Vessel-Targeted Liposomal BPD-MA 324
5. Usefulness of Antiangiogenic PDT with Neovessel-Targeted Liposomes 327
6. Concluding Remarks 329
References 329
17. Controlling the In Vivo Activity of Wnt Liposomes
1. Introduction
2. Materials， Methods， and Results
3. Concluding Remarks
References
18. Convection-Enhanced Delivery of Liposomes to Primate Brain 349
1. Introduction
2. Liposome Preparation
3. Quantification of liposome-Entrapped Gadoteridol by Magnetic Resonance Imaging
4. Experimental Subjects
5. Infusion Catheter Design and Infusion Procedure
6. Distribution of Liposomes Within Anatomic Structures of the Primate Brain
7. Volumetric Calculations of Liposomal Distribution in Primate CNS
8. Future and Outlook of CED to Brain
Acknowledgment
References
19. Hemoglobin-Vesicles as an Artificial Oxygen Carrier
1. Introduction: Encapsulated Hemoglobin as an Artificial Oxygen Carrier
2. Encapsulation of Concentrated Hb in Liposomes
3. Source of Hb and Its Purification
4. Regulation of Oxygen Affinity
5. Structural Stabilization of Liposome-Encapsulated Hb
6. Blood Compatibility of LEH and HbV
7. Regulation of Osmotic Pressure and Suspension Rheology to Mimic and Overwhelm the Function of Blood
8. Concluding Remarks
References
Author Index
Subject Index
