Liposomes : methods and protocols. v. 2, Biological membrane models / edited by Volkmar Weissig. — New York ; London : Humana, c2010. – (58.17/M592/v.606)

Contents

    Contents
    
    Preface
    Contributors
    1 Utilization of Liposomes for Studying Drug Transfer and Uptake
    2 The Use of Liposomes in the Study of Drug Metabolism: A Method to Incorporate the Enzymes of the Cytochrome P450 Monooxygenase System into Phospholipid， Bilayer Vesicles
    3 Use of Liposomes to Study Cellular Osmosensors
    4 Studying Mechanosensitive Ion Channels Using Liposomes
    5 Studying Amino Acid Transport Using Liposomes
    6 Use of Liposomes for Studying Interactions of Soluble Proteins with Cellular Membranes
    7 Liposomal Reconstitution of Monotopic Integral Membrane Proteins
    8 The Reconstitution of Actin Polymerization on Liposomes
    9 Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for the Study of Lipid Domains under Physiological Ionic-Strength Conditions
    10 Visualization of Lipid Domain-Specific Protein Sorting in Giant Unilamellar Vesicles
    11 Biosynthesis of Proteins Inside Liposomes
    12 Study of Respiratory Cytochromes in Liposomes 147
    13 Use of Liposomes to Evaluate the Role of Membrane Interactions on Antioxidant Activity
    14 Studying Colloidal Aggregation Using Liposomes
    15 Assessment of Liposome-Cell Interactions
    16 Methods to Monitor Liposome Fusion， Permeability， and Interaction with Cells
    17 The Use of Isothermal Titration Calorimetry to Study Multidrug Transport Proteins in Liposomes
    18 Studying Lipid Organization in Biological Membranes Using Liposomes and EPR Spin Labeling
    19 Membrane Translocation Assayed by Fluorescence Spectroscopy 271
    20 Interaction of Lipids and Ligands with Nicotinic Acetylcholine Receptor Vesicles Assessed by Electron Paramagnetic Resonance Spectroscopy 291
    21 Environmental Scanning Electron Microscope Imaging of Vesicle Systems 319
    22 Freeze-Fracture Electron Microscopy on Domains in Lipid Mono- and Bilayer on Nano-Resolution Scale
    23 Atomic Force Microscopy for the Characterization of Proteoliposomes
    24 Method of Simultaneous Analysis of Liposome Components Using HPTLC/FID 363
    25 Viscometric Analysis of DNA-Lipid Complexes
    26 Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes 385
    27 Fluorescence Resonance Energy Transfer-Based Analysis of Lipoplexes
    28 Analysis of Lipoplex Structure and Lipid Phase Changes
    29 Fluorescence Methods for Evaluating Lipoplex-Mediated Gene Delivery 425
    30 FRET Imaging of Cells Transfected with siRNA/Liposome Complexes 439
    31 Spectral Bio-Imaging and Confocal Imaging of the Intracellular Distribution of Lipoplexes 457
    32 Techniques for Loading Technetium-99m and Rhenium-186/188 Radionuclides into Pre-formed Liposomes for Diagnostic Imaging and Radionuclide Therapy
    33 Fluorescence Correlation Spectroscopy for the Study of Membrane Dynamics and Organization in Giant Unilamellar Vesicles
    34 Liposome Biodistribution via Europium Complexes
    35 Biosensor-Based Evaluation of Liposomal Binding Behavior
    36 Use of Liposomes to Study Vesicular Transport
    Index