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Peptidomics : methods and protocols / edited by Mikhail Soloviev. — Totowa, N.J. : Humana, 2010. – (58.17/M592/v.615) |
Contents
Contents
Preface
Contributors
SECTION I INTRODUCTION
1. Peptidomics: Divide et Impera
SECTION II FROM BACTERIA TO MEN
2. Performing Comparative Peptidomics Analyses of Salmonella from Different Growth Conditions 13
3. Approaches to Identify Endogenous Peptides in the Soil Nematode Caenorhabditis elegans 29
4. Mass Spectrometric Analysis of Molluscan Neuropeptides
5. Monitoring Neuropeptides In Vivo via Microdialysis and Mass Spectrometry
6. Protocols for Peptidomic Analysis of Spider Venoms 75
7. Purification and Characterization of Biologically Active Peptides from Spider Venoms 87
8. MALDI-TOF Mass Spectrometry Approaches to the Characterisation of Insect Neuropeptides
9. Direct MALDI-TOF Mass Spectrometric Peptide Profiling of Neuroendocrine Tissue of Drosophila
10. Direct Peptide Profiling of Brain Tissue by MALDI-TOF Mass Spectrometry
11. Peptidomic Analysis of Single Identified Neurons
12. Identification and Analysis of Bioactive Peptides in Amphibian Skin Secretions
13. An Efficient Protocol for DNA Amplification of Multiple Amphibian Skin Antimicrobial Peptide cDNAs
14. Combined Peptidomics and Genomics Approach to the Isolation of Amphibian Antimicrobial Peptides
15. Identification and Relative Quantification of Neuropeptides from the Endocrine Tissues
16. Peptidome Analysis of Mouse Liver Tissue by Size Exclusion Chromatography Prefractionation
17. Rat Brain Neuropeptidomics: Tissue Collection， Protease Inhibition， Neuropeptide Extraction， and Mass Spectrometric Analysis
18. Quantitative Neuroproteomics of the Synapse
19. Peptidomics Analysis of Lymphoblastoid Cell lines
20. Peptidomics: Identification of Pathogenic and Marker Peptides
SECTION III TOOLS AND APPROACHES
21. Peptidomic Approaches to the Identification and Characterization of Functional Peptides in Hydra
22. Immunochemical Methods for the Peptidomic Analysis of Tachykinin Peptides and Their Precursors
23. Affinity Peptidomics: Peptide Selection and affinity Capture on Hydrogels and Microarrays
24. In Situ Biosynthesis of Peptide Arrays
25. Bioinformatic Approaches to the Identification of Novel Neuropeptide Precursors
26. Bioinformatic Identification of Plant Peptides
Index
