Epidermal cells : methods and protocols / edited by Kursad Turksen. — 2nd ed. — New York : Humana Press, c2010. – (58.17/M592/v.585) |
Contents
Contents
Preface
Contributors
1. Isolation of Adult Mouse Stem Keratinocytes Using Magnetic Cell Sorting (MACS)
2. Functional Investigations of Keratinocyte Stem Cells and Progenitors at a Single-Cell Level Using Multiparallel Clonal Microcultures
3. Growth and Stratification of Epithelial Cells in Minimal Culture Conditions
4. Matched Cultures of Keratinocytes and Fibroblasts Derived from Normal and NER-Deficient Mouse Models
5. Establishment of Spontaneously Immortalized Keratinocyte Lines from Wild-Type and Mutant Mice
6. Study of Epidermal Differentiation in Human Keratinocytes Cultured in Autocrine Conditions
7. Directed Differentiation of Human Embryonic Stem Cells to Epidermal Progenitors
8. Expression and Analysis of Exogenous Proteins in Epidermal Cells 93
9. Using siRNA Knockdown in HaCaT Cells to Study Transcriptional Control of Epidermal Proliferation Potential
10. RNA Interference in Keratinocytes and an Organotypic Model of Human Epidermis 127
11. Scanning for Transcription Factor Binding by a Variant EMSA 147
12. Chromatin Immunoprecipitation for Identifying Transcription Factor Targets in Keratinocytes
13. Gene Expression Profiling of Mouse Epidermal Keratinocytes
14. Analysis of Tissue-Specific Gene Expression Using Laser Capture Microdissection
15. Comprehensive Transcriptional Profiling of Human Epidermis, Reconstituted Epidermal Equivalents, and Cultured Keratinocytes Using DNA Microarray Chips 193
16. Molecular Profiling of the Epidermis: A Proteomics Approach 225
17. Detection of Gene Expression in Embryonic Tissues and Stratified Epidermis by In Situ Hybridization 253
18. Embryonic Mammary Anlagen Analysis Using Immunolabelling of Whole Mounts
19. Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis 271
20. Tetracycline-Regulated Gene Expression in Transgenic Mouse Epidermis 287
21. A Versatile Murine 3D Organotypic Model to Evaluate Aspects of Wound Healing and Epidermal Organization 303
22. Optical and Biochemical Dissection of Connexin and Disease-Linked Connexin Mutants in 3D Organotypic Epidermis 313
23. Cytokine Release in Tissue-Engineered Epidermal Equivalents After Prolonged Mechanical Loading
24. Three-Dimensional Human Tissue Models of Wounded Skin 345
25. In Vivo Transplantation of Genetically Modified Mouse Embryonic Epidermis 361
26. A Transplant Model for Human Epidermal Skin Regeneration 369
27. Identification of Epithelial Stem Cells In Vivo and In Vitro Using Keratin 19 and BrdU
28. Isolation and Culture of Hair Follicle Pluripotent Stem (hfPS) Cells and Their Use for Nerve and Spinal Cord Regeneration
29. Limiting Dilution Analysis of Murine Epidermal Stem Cells Using an In Vivo Regeneration Assay
Subject Index 433