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Heterologous expression of membrane proteins : methods and protocols / edited by Isabelle Mus-Veteau. — Totowa, N.J. : Humana, c2010. – (58.17/M592/v.601)

Contents

    Contents
    
    Preface
    Contributors
    1 Heterologous Expression of Membrane Proteins for Structural Analysis
    2 Production of Membrane Proteins in Escherichia coli and Lactococcus lactis
    3 Mammalian Membrane Receptors Expression as Inclusion Bodies in Escherichia coli
    4 Expression of Membrane Proteins at the Escherichia coli Membrane for Structural Studies
    5 Membrane Protein Expression in Lactococcus lactis
    6 Heterologous Expression of Human Membrane Receptors in the Yeast Saccharomyces cerevisiae 87
    7 Mammalian Membrane Protein Expression in Baculovirus-Infected Insect Cells 105
    8 Expression of membrane proteins in Drosophila melanogaster S2 Cells: Production and Analysis of a EGFP-Fused G Protein-Coupled Receptor as a Model 119
    9 Membrane Protein Expression in the Eyes of Transgenic Flies 135
    10 Expression of Mammalian Membrane Proteins in Mammalian Cells Using Semliki Forest Virus Vectors 149
    11 Membrane Protein Expression in Cell-Free Systems
    12 Practical Considerations of Membrane Protein Instability during Purification and Crystallisation 187
    13 Membrane Protein Solubilization 205
    14 Amphipols and Fluorinated Surfactants: Two Alternatives to Detergents
    for Studying Membrane Proteins In vitro
    15 Heterologous Expression and Affinity Purification of Eukaryotic Membrane Proteins in View of Functional and Structural Studies: The Example of the Sarcoplasmic Reticulum Ca2+-ATPase 247
    Index 269