Live cell imaging : methods and protocols / edited by Dmitri B. Papkovsky. — New York : Humana, c2010. – (58.17/M592/v.591)

Contents

        Contents
    
    Preface
    Contributors
    PART I GENERAL PRINCIPLES AND OVERVIEW
    1. Instrumentation for Live-Cell Imaging and Main Formats
    2. Labels and Probes for Live Cell Imaging Overview and Selection Guide
    3. Live Cell Imaging: An Industrial Perspective
    PART II IMAGING TECHNIQUES， PROBES， AND APPLICATIONS 67
    4. Design of Fluorescent Fusion Protein Probes 69
    5. Synthetic Fluorescent Probes for Imaging of Peroxynitrite and Hypochlorous Acid in Living Cells 93
    6. Photo-Activatable Probes for the Analysis of Receptor Function in Living Cells 105
    7. The Application of Fluorescent Probes for the Analysis of Lipid Dynamics During Phagocytosis
    8. Imaging of Mitotic Cell Division and Apoptotic Intra-Nuclear Processes in Multicolor 135
    9. Manipulation of Neutrophil-Like HL-60 Cells for the Study of Directed Cell Migration 147
    10. A Method for Analyzing Protein-Protein Interactions in the Plasma Membrane of Live B Cells by Fluorescence Resonance Energy Transfer Imaging as Acquired by Total Internal Reflection Fluorescence Microscopy 159
    11. Sample Preparation for STED Microscopy
    12. Two-Photon Permeabilization and Calcium Measurements in Cellular Organelles 201
    13. Imaging and Analysis of Three-Dimensional Cell Culture Models
    14. Long-Term Imaging in Microfluidic Devices
    15. Monitoring of Cellular Responses to Hypoxia
    16. Imaging of Cellular Oxygen and Analysis of Metabolic Responses of Mammalian Cells 257
    17. Analysis of Mitochondrial pH and Ion Concentrations 275
    18. Live Cell Imaging Analysis of Receptor Function
    19. Subcellular Dynamic Imaging of Protein-Protein Interactions in Live Cells by Bioluminescence Resonance Energy Transfer 325
    20. Quantitative Analysis of Membrane Potentials
    21. Image Correlation Spectroscopy to Define Membrane Dynamics 353
    Subject Index 365