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Chromatin immunoprecipitation assays : methods and protocols / edited by Philippe Collas. — New York ; London : Humana, c2009. – (58.17/M592/v.567)

Contents

    Contents
    
    Contributors
    1. The State-of-the-Art of Chromatin Immunoprecipitation
    2. Characterization and Quality Control of Antibodies Used in ChIP Assays 27
    3. The Fast Chromatin Immunoprecipitation Method 45
    4. μChIP: Chromatin Immunoprecipitation for Small Cell Numbers 59
    5. Fish'n ChIPs: Chromatin Immunoprecipitation in the Zebrafish Embryo 75
    6. Epitope Tagging of Endogenous Proteins for Genome-Wide Chromatin Immunoprecipitation Analysis 87
    7. Flow Cytometric and Laser Scanning Microscopic Approaches in Epigenetics Research 99
    8. Serial Analysis of Binding Elements for Transcription Factors
    9. Modeling and Analysis of ChIP-Chip Experiments
    10. Use of SNP-Arrays for ChIP Assays: Computational Aspects
    11. DamID: A Methylation-Based Chromatin Profiling Approach 155
    12. Chromosome Conformation Capture (from 3C to 5C) and Its ChIP-Based Modification 171
    13. Determining Spatial Chromatin Organization of Large Genomic Regions Using 5C Technology 189
    14. Analysis of Nascent RNA Transcripts by Chromatin RNA Immunoprecipitation 215
    15. Methyl DNA Immunoprecipitation 237
    16. Immunoprecipitation of Methylated DNA
    Index