High throughput screening : methods and protocols / edited by William P. Janzen and Paul Bernasconi. — 2nd ed. — Dordrecht ; London : Humana Press, c2009. – (58.17/M592/v.565) |
Contents
Contents
Preface
Contributors
1. Design and Implementation of High-Throughput Screening Assays
2. Creation of a Small High-Throughput Screening Facility
3. Informatics in Compound Library Management
4. Statistics and Decision Making in High-Throughput Screening
5. Enzyme Assay Design for High-Throughput Screening
6. Application of Fluorescence Polarization in HTS Assays
7. Screening G Protein-Coupled Receptors: Measurement of Intracellular Calcium Using the Fluorometric Imaging Plate Reader
8. High-Throughput Automated Confocal Microscopy Imaging Screen of a Kinase-Focused Library to Identify p38 Mitogen-Activated Protein Kinase Inhibitors Using the GE InCell 3000 Analyzer
9. Recent Advances in Electrophysiology-Based Screening Technology and the Impact upon Ion Channel Discovery Research 187
10. Automated Patch Clamping Using the QPatch
11. High-Throughput Screening of the Cyclic AMP-Dependent Protein Kinase (PKA) Using the Caliper Microfluidic Platform
12. Use of Primary Human Cells in High-Throughput Screens
Index