Bioluminescence : methods and protocols / edited by Preston B. Rich, Christelle Douillet. — 2nd ed. — New York : Humana Press, 2009. – (58.17/M592/v.574)

Contents

    Contents
    
    Contributors
    1. Microfluidic Techniques for the Analysis of Bacterial Chemotaxis
    2. Prokaryotic Phototaxis 25
    3. Photoorientation in Photosynthetic Flagellates
    4. Dictyostelium Slug Phototaxis
    5. Electrotaxis and Wound Healing: Experimental Methods to Study Electric Fields as a Directional Signal for Cell Migration 77
    6. Chemotropism During Yeast Mating 99
    7. Group Migration and Signal Relay in Dictyostelium 111
    8. Quantitative Analysis of Distal Tip Cell Migration in C. elegans 125
    9. Inflammation and wound healing in drosophila
    10. Neutrophil motility in vivo using zebrafish
    11. Chemotaxis in Neutrophil-Like HL-60 Cells 167
    12. Chemokine Receptor Dimerization and Chemotaxis 179
    13. Intravital Two-Photon Imaging of Adoptively Transferred B Lymphocytes in Inguinal Lymph Nodes 199
    14. Breast Cancer Cell Movement: Imaging Invadopodia by TIRF and IRM Microscopy 209
    15. In Vivo Assay for Tumor Cell Invasion
    16. Quantitative Studies of Neuronal Chemotaxis in 3D
    17. Assays for Chemotaxis and Chemoattractant-Stimulated TorC2 Activation and PKB Substratc Phosphorylation in Dictyostelium 255
    18. Biochemical Responses to Chemoattractants in Dictyostelium: Ligand-Receptor Interactions and Downstream Kinase Activation
    19. Quantifying In Vivo Phosphoinositide Turnover in Chemotactically Competent Dictyostelium Cells 283
    20. In Vivo Measurements of Cytosolic Calcium in Dictyostelium discoideum
    21. Chemokine Receptor Signaling and HIV Infection
    22. Spatiotemporal Stimulation of Single Cells Using Flow Photolysis
    23. Spatiotemporal Regulation of Ras-GTPases During Chemotaxis
    24. FRAP Analysis of Chemosensory Components of Dictyostelium
    25. Monitoring Dynamic GPCR Signaling Events Using Fluorescence Microscopy， FRET Imaging， and Single-Molecule Imaging 371
    26. Imaging Actin Cytoskeleton Dynamics in Dictyostelium Chemotaxis 385
    27. Analysis of Actin Assembly by In Vitro TIRF Microscopy 401
    28. Single-Molecule Imaging Techniques to Visualize Chemotactic Signaling Events on the Membrane of Living Dictyostelium Cells
    29. Imaging B-Cell Receptor Signaling by Single-Molecule Techniques
    30. Light Microscopy to Image and Quantify Cell Movement
    31. Mathematics of Experimentally Generated Chemoattractant Gradients
    32. Modeling Spatial and Temporal Dynamics of Chemotactic Networks 489
    33. Computational Modeling of Signaling Networks for Eukaryotic Chemosensing 507
    Index