Peptide microarrays : methods and protocols / edited by Marina Cretich, Marcella Chiari. — Dordrecht : Springer, 2009. – (58.17/M592/v.570)

Contents

    Contents
    
    Preface
    Contributors
    SECTION I: INTRODUCTION
    1 Exploring and Profiling Protein Function with Peptide Arrays
    2 Peptide Arrays for Enzyme Profiling 19
    3 Using Peptide Array to Identify Binding Motifs and Interaction Networks for Modular Domains 67
    4 Molecular Simulations of Peptides: A Useful Tool for the Development of New Drugs and for the Study of Molecular Recognition 77
    SECTION II: APPLICATIONS
    5 Synthesis of Peptide Arrays Using SPOT-Technology and the CelluSpots-Method 157
    6 Rapid Identification of Linear Protein Domain Binding Motifs Using Peptide SPOT Arrays 175
    7 Characterization of Kinase Target Phosphorylation Consensus Motifs Using Peptide SPOT Arrays 187
    8 CelluSpots: A Reproducible Means of Making Peptide Arrays for the Determination of SH2 Domain Binding Specificity 197
    9 High-Density Peptide Microarrays for Reliable Identification of Phosphorylation Sites and Upstream Kinases 203
    10 Epitope Mapping of Human Chromogranin A by Peptide Microarrays 221
    11 Antimicrobial Peptide Arrays for Detection of Inactivated Biothreat Agents 233
    12 Mapping Functional Prion-Prion Protein Interaction Sites Using Prion Protein Based Peptide-Arrays 257
    13 A Designed Peptide Chip: Protein Fingerprinting Technology with a Dry Peptide Array and Statistical Data Mining 273
    SECTION III: TECHNOLOGICAL ADVANCEMENTS
    14 Peptide Microarrays on Bisphenol A Polycarbonate 287
    15 Self-Assembly of PNA-Encoded Peptides into Microarrays 299
    16 A Novel Combinatorial Approach to High-Density Peptide Arrays 309
    17 Polypyrrole-Peptide Microarray for Biomolecular Interaction Analysis by SPR Imaging 317
    18 The Peptide Microarray-Based Assay for Kinase Functionality and Inhibition Study 329
    19 An Advanced Application of Protein Microarrays: Cell-Based Assays for Functional Genomics 339
    20 Profiling the Autoantibody Repertoire by Screening Phage-Displayed Human cDNA Libraries 353
    SECTION IV: SOFTWARE/WEB TOOLS AND DATA ANALYSIS
    21 Visualisation and Pre-processing of Peptide Microarray Data 373
    22 Web-Based Design of Peptide Microarrays Using μPepArray Pro 391
    23 Qualitative and Quantitative Analysis of Peptide Microarray Binding Experiments Using SVM-PEPARRAY 403
    24 PASE: A Web-Based Platform for Peptide/Protein Microarray Experiments 413
    Index 431