Cytoskeleton methods and protocols / edited by Ray H. Gavin. — 2nd ed. — New York ; London : Humana Press, c2009. – (58.17/M592/v.586)

Contents

    Contents
    
    Preface
    Contributors
    PART I LIVE - CELL IMAGING OF THE CYTOSKELETON
    1 High Resolution Multimode Light Microscopy of Cell Migration Long-Term Imaging and Analysis
    2 Imaging the Cytoskeleton in Live Xenopus laevis Embryos
    3 Live-Cell Imaging of the Cytoskeleton and Mitochondria-Cytoskeleton
    Interactions in Budding Yeast
    PART II DYNAMICS OF ACTIN FILAMENTS AND MICROTUBULES
    4 Jasplakinolide: An Actin-Specific Reagent that Promotes Actin Polymerization
    5 Analysis of Centrosome Function and Microtubule Dynamics by Time-Lapse Microscopy in Xenopus Egg Extracts
    PART III FLUORESCENCE MICROSCOPY PROTOCOLS FOR INVESTIGATING CYTOSKELETON COMPONENTS
    6 Quantitative Fluorescence Microscopy Techniques 117
    7 A FRET-Based Approach for Studying Conformational Changes of a Cytoskeleton-Related Tumor Suppressor Molecule 143
    8 Sample Preparation for Fluorescence Imaging of the Cytoskeleton in Fixed and Living Plant Roots 157
    9 Imaging of the Cytoskeleton and Mitochondria in Fixed Budding Yeast Cells 171
    PART IV ELECTRON MICROSCOPY PROTOCOLS FOR IMAGING THE CYTOSKELETON
    10 Imaging Cytoskeleton Components by Electron Microscopy 187
    11 Purification and Localization of Intraflagellar Transport Particles and Polypeptides
    12 High Pressure Freezing， Electron Microscopy， and Immuno-Electron Microscopy of Tetrahymena thermophila Basal Bodies 227
    13 Strategies for Imaging Microtubules in Plant Cells 243
    PART V ANALYSIS OF CELL AND ORGANELLE MOTILITY
    14 A Rapid Tracking Method for the Quantitative Analysis of Organelle Streaming Velocity
    15 Melanosome Motility in Fish Retinal Pigment Epithelial Cells 275
    16 Analysis of Properties of Cilia Using Tetrahymena thermophila 283
    17 Application of Cell Traction Force Microscopy for Cell Biology Research 301
    18 2D and 3D Quantitative Analysis of Cell Motility and Cytoskeletal Dynamics 315
    19 Detergent-Extracted Models for the Study of Cilia or Flagella 337
    PART VI GENETIC AND PROTEOMICS APPROACHES FOR CYTOSKELETON PROTEIN FUNCTION
    20 The Analysis of Intermediate Filament Dynamics Using Transfections and Cell Fusions
    21 Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila
    22 Proteomic Tools for the Analysis of Cytoskeleton Proteins 375
    Index