DNA topoisomerases : methods and protocols / edited by Duncan J. Clarke. — New York, N.Y. : Humana Press, c2009. – (58.17/M592/v.582) |
Contents
Contents
Contributors
1. Introduction: Emerging Themes in DNA Topoisomerase Research
2. Analysis of DNA Topoisomers, Knots, and Catenanes by Agarose Gel Electrophoresis
3. Two-Dimensional Agarose Gel Electrophoresis of DNA Topoisomers
4. Cleavage of Plasmid DNA by Eukaryotic Topoisomerase II
5. Assays for the Preferential Binding of Human Topoisomerase I to Supercoiled DNA
6. Monitoring the Topoisomerase II DNA Gate Conformational Change with Fluorescence Resonance Energy Transfer
7. Single-Molecule Magnetic Tweezers Studies of Type IB Topoisomerases
8. Dissolution of Double Holliday Junctions by the Concerted Action of BLM and Topoisomerase IIIα
9. ChIP-on-Chip Analysis of DNA Topoisomerases
10. Analyzing Top2 Distribution on Yeast Chromosomes by Chromatin Immunoprecipitation
11. Binding of DNA Topoisomerases I and II to Replication Origins
12. Cytometric Assessment of DNA Damage Induced by DNA Topoisomerase Inhibitors
13. Analysis of the Topoisomerase II-Dependent Decatenation G2 Checkpoint and Checkpoint Kinases in Human Cells
14. Assaying Topoisomerase II Checkpoints in Yeast
15. Cytological Analysis of Chromosome Structural Defects that Result from Topoisomerase II Dysfunction
16. Top2 SUMO Conjugation in Yeast Cell Lysates
17. Analysis of SUMOylation of Topoisomerase IIα with Xenopus Egg Extracts
18. The Dynamics of DNA Topoisomerase IIα in Living Cells
19. Depletion and Mutation of Topoisomerase II in Animal Cells
Subject Index