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新书资源(2010年4月)

DNA topoisomerases : methods and protocols / edited by Duncan J. Clarke. — New York, N.Y. : Humana Press, c2009. – (58.17/M592/v.582)

Contents

    Contents
    
    Contributors
    1. Introduction: Emerging Themes in DNA Topoisomerase Research
    2. Analysis of DNA Topoisomers, Knots, and Catenanes by Agarose Gel Electrophoresis
    3. Two-Dimensional Agarose Gel Electrophoresis of DNA Topoisomers
    4. Cleavage of Plasmid DNA by Eukaryotic Topoisomerase II
    5. Assays for the Preferential Binding of Human Topoisomerase I to Supercoiled DNA
    6. Monitoring the Topoisomerase II DNA Gate Conformational Change with Fluorescence Resonance Energy Transfer
    7. Single-Molecule Magnetic Tweezers Studies of Type IB Topoisomerases
    8. Dissolution of Double Holliday Junctions by the Concerted Action of BLM and Topoisomerase IIIα
    9. ChIP-on-Chip Analysis of DNA Topoisomerases
    10. Analyzing Top2 Distribution on Yeast Chromosomes by Chromatin Immunoprecipitation
    11. Binding of DNA Topoisomerases I and II to Replication Origins
    12. Cytometric Assessment of DNA Damage Induced by DNA Topoisomerase Inhibitors
    13. Analysis of the Topoisomerase II-Dependent Decatenation G2 Checkpoint and Checkpoint Kinases in Human Cells
    14. Assaying Topoisomerase II Checkpoints in Yeast
    15. Cytological Analysis of Chromosome Structural Defects that Result from Topoisomerase II Dysfunction
    16. Top2 SUMO Conjugation in Yeast Cell Lysates
    17. Analysis of SUMOylation of Topoisomerase IIα with Xenopus Egg Extracts
    18. The Dynamics of DNA Topoisomerase IIα in Living Cells
    19. Depletion and Mutation of Topoisomerase II in Animal Cells
    Subject Index