首页 > 新书资源
新书资源(2010年2月)

Monoclonal antibodies in biology and biotechnology : theoretical and practical aspects / by Kenneth C. McCullough, Raymond E. Spier. — Cambridge ; New York : Cambridge University Press, 1990. – (58.6913/M133)

Contents

    Contents
    
    1. Introduction: the immune response
    1.1. Introduction
    1.2. A history of the early development of immunology
    1.3. The antibodies
    1.4. The immune response
    1.5. Affinity of antibodies and the immune response
    1.6. Antibody response
    1.7. Monoclonal antibodies (MAb)
    1.8. Summary
    2 Making hybridomas (hybridoma technology)
    2.1. Basic requirements
    2.2. The myeloma cell
    2.3. The production of hybridomas from myeloma cells
    2.4. Myeloma cells available
    2.5. Generation of hybridoma cell lines
    2.6. A practical guide to generating hybridoma cell lines
    2.7. Types of hybridoma cell line
    2.8. Summary
    3 Factors affecting successful hybridoma production
    3.1. Efficacy of immunisation
    3.2. Source of myeloma cells
    3.3. Source of PEG
    3.4. Fusion ratio
    3.5. Source of media and media supplements
    3.6. Physical factors
    3.7. Growth factors
    3.8. Culturing protocols
    3.9. Summary
    4 Selection of monoclonal antibody-secreting hybridoma cell lines
    4.1. Screening of polyclonal hybridomas
    4.2. Cloning
    4.3. Cloning immediately after fusion
    4.4. Determination of monoclonality
    4.5. Culturing of monoclonal antibody-secreting hybridoma cell lines
    4.6. Summary
    5 The large-scale production of monoclonal antibodies in vitro
    5.1. Introduction
    5.2. The cell population
    5.3. Culture media
    5.4. A survey of the advantages and disadvantages of techniques based on dense cell systems
    5.5. The influence of controllable parameters on monoclonal antibody production by hybridoma cells in culture
    5.6. The technologies used to produce large quantities of monoclonal antibodies from cells held in culture
    5.7. Conclusions
    6 In conclusion: the diversity in application of monoclonal antibodies and hybridoma technology
    6.1. Research applications
    6.2. Diagnostic applications of MAb
    6.3. Preparative uses of MAb
    6.4. Drug targetting using MAb
    6.5. Prophylactic and therapeutic uses of MAb
    6.6. Idiotopes and idiotypes
    6.7. Summary
    References
    Index