Genetic dissection of neural circuits and behavior / edited by Stephen F. Goodwin. — Amsterdam : Elsevier, c2009. – (58.14/A244/v.65)

Contents

    Contents
    
    Contributors vii
    1 Genetic Dissection of Neural Circuits and Behavior in Mus musculus 1
    I. Conventional Gene Targeting Strategies 2
    II. Spatially Restricted Gene Manipulation 5
    III. The Use of Dominant Negative Inhibitors to Manipulate Gene Function 10
    IV. Conditional Manipulation of Gene Function 11
    V. Pharmacogenetic Approaches 16
    VI. Optogenetic Approaches 22
    VII. Visualization of Brain Structure and Function 24
    VIII. The Challenges Facing Genetic Approaches in the Mouse 28
    References 32
    2 Worm Watching: Imaging Nervous System Structure and Function in Caenorhabditis elegans 39
    I. Introduction 40
    II. Real-Time Indicators of Neuronal Activity
    III. Monitoring Intracellular Calcium 45
    IV. Imaging Presynaptic Terminals 49
    V. Imaging Postsynaptic Compartments 54
    VI. Imaging Proxies for Neuropeptide Secretion
    VII. Bimolecular Fluorescence Complementation in the Worm 60
    VIII. Methods for Immobilizing the Worm 61
    IX. Femtosecond Lasers and Nanosurgery on the Worm 65
    X. Designing the Ideal Optical Probe 67
    XI. Conclusions and Prospects 70
    References 70
    3 Mapping and Manipulating Neural Circuits in the Fly Brain 79
    I. Introduction 80
    II. Spatial Targeting of Neuron Types 86
    III. Imaging Neurons 92
    IV. Functional Imaging: Watching Neuronal Activity
    V. Control of Neural Activity 100
    VI. Quantitative Behavioral Assays 113
    VII. Conclusions 118
    References 121
    4 A Network of G-Protein Signaling Pathways Control Neuronal Activity in C. elegans 145
    I. Introduction 146
    II. Mapping Intracellular Neural Networks within C. elegans Motorneurons 151
    References 178
    Index 193
    Color Plate Section at the end of the Book