Protein engineering handbook. v. 1 / edited by Stefan Lutz and Uwe T. Bornscheuer. — Weinheim : Wiley-VCH, c2009. – (82.95073/P967/v.1)

Contents

    CONTENTS
    
    Volume 1
    1 Guidelines for the functional analysis of engineered and mutant enzymes
    2 Engineering enantioselectivity in enzyme-catalyzed reactions
    3 Mechanism and catalytic promiscuity: emerging mechanistic principles for identification and manipulation of catalytically promiscuous enzymes
    4 Φ-value analysis of protein folding transition states
    5 protein folding and solubility: pathways and high-throughput assays
    6 protein dynamics and the evolution of novel protein function
    7 gaining insight into enzyme function through correlation with protein motions
    8 structural frameworks suitable for engineering
    9 microbes and enzymes: recent trends and new directions to expand protein space
    10 Inteins in protein engineering
    11 from prospecting to product-industrial metagenomics is coming of age
    12 computational protein design
    13 assessing and exploiting the persistence of substrate ambiguity in modern protein catalysts
    14 designing programmable protein switches
    15 the cyclization of peptides and proteins with Inteins
    Volume 2
    16 a method for rapid directed evolution
    17 Evolution of enantioselective bacillus subtilis lipase
    18 Circular permutation of proteins
    19 Incorporating synthetic oligonucleotides via gene reassembly(ISOR): a versatile tool for generating targeted libraries
    20 Protein engineering by structure-guided SCHEMA recombination
    21 Chimeragenesis in protein engineering
    22 Protein generation using a reconstituted system
    23 Equipping in vivo selection systems with tunable stringency
    24 Protein engineering by phage display
    25 Screening methodologies for glycosidic bond formation
    26 Yeast surface display in protein engineering and analysis
    27 In vitro compartmentalization (IVC) and other high-throughput screens of enzyme libraries
    28 Colorimetric and fluorescence-based screening
    29 Confocal and conventional fluorescence-based high throughput screening in protein engineering
    30 Alteration of substrate specificity and stereoselectivity of lipases and esterases
    31 Altering enzyme substrate and cofactor specificity via protein engineering
    32 Protein engineering of modular polyketide synthases
    33 Cyanophycin Synthetases
    34 Biosynthetic pathway engineering strategies
    35 Natural polyester-related proteins: structure， function evolution and engineering
    36 Bioengineering of sequence-repetitive polypeptides: synthetic routes to protein-based materials of novel structure and function
    37 Silk proteins-biomaterials and bioengineering
    Index