首页 > 新书资源
新书资源(2009年12月)

Handbook of cryo-preparation methods for electron microscopy / edited by Annie Cavalier, Daniele Spehner, Bruno M. Humbel. — Boca Raton : CRC Press, c2009. – (58.1714/H236)

Contents

    CONTENTS
    
    Introduction
    Abbreviations
    Chapter 1 Vitreous Water
    Part I - Cryo-Fixation Methods
    Chapter 2 Slam-Freezing, Metal-Mirror Freezing
    Chapter 3 Plunge-Freezing (Holey Carbon Method)
    Chapter 4 Controlled Vitrification
    Chapter 5 BAL-TEC HPM 010 High-Pressure Freezing Machine
    Chapter 6 High-Pressure Freezing LEICA EMPACT
    Part II - Cryo-Electron Microscopy
    Chapter 7 Frozen-Hydrated Macromolecules for Structural Analysis
    Chapter 8 Two-Dimensional Crystals
    Chapter 9 Cryo-Negative Staining
    Chapter 10 Vitrification of Dynamic Microtubules
    Chapter 11 CEMOVIS: Cryo-Electron Microscopy of Vitreous Sections
    Chapter 12 Cryo-Electron Tomography
    Part III - Low-Temperature Embedding
    Chapter 13 Freeze-Substitution
    Chapter 14 Cryo-Fixation, Freeze-Substitution, Rehydration and Tokuyasu Cryo- Sectioning
    Chapter 15 Freeze-Drying and Embedding of Biological Material
    Part IV - Freeze-Fracture and Metal Shadowing
    Chapter 16 The Shadow of Hydrated Biological Specimens
    Chapter 17 Cryo-Fracture of Self-Assembled Systems in Organic Solvent
    Part V - Analysis
    Chapter 18 Progressive Lowering of Temperature for Immunolabeling and in situ Hybridization
    Chapter 19 Cryo-Sectioning According to Tokuyasu
    Chapter 20 Cryo-Preparation Procedures for Elemental lmaging by SIMS and EFTEM
    Chapter 21 Correlative Light and Electron Microscopy
    Chapter 22 SDS-digested Freeze-Fracture Replica Labeling (SDS-FRL)
    Chapter 23 Immunolabeling of Ultrathin Sections with Enlarged 1 nm Gold or QDots
    Chapter 24 3-D Electron Tomography of Cells and Organelles
    Final Considerations
    Glossary
    Index