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Handbook of nucleic acid purification / edited by Dongyou Liu. — Boca Raton, Fla. : London : CRC ; Taylor & Francis [distributor], 2009. – (58.17425073/H236) |
Contents
Contents
Preface ix
Editor xi
Contributors xiii
Chapter 1 Nucleic Acids: Structures， Functions， and Applications 1
PART I Purification of Nucleic Acids from Viruses
Chapter 2 Isolation of Viral DNA from Cultures 23
Chapter 3 Isolation of Viral RNA from Cultures 41
Chapter 4 Preparation of Viral Samples for Direct Molecular Applications 61
PART II Purification of Nucleic Acids from Bacteria
Chapter 5 Isolation of Bacterial DNA from Cultures 85
Chapter 6 Isolation of Bacterial RNA from Cultures 107
Chapter 7 Isolation of Plasmids 127
Chapter 8 Preparation of Bacterial Samples for Direct Molecular Applications 147
PART III Purification of Nucleic Acids from Fungi
Chapter 9 Isolation of Nucleic Acids from Yeasts 171
Chapter 10 Isolation of Nucleic Acids from Filamentous Fungi 191
Chapter 11 Preparation of Fungal Specimens for Direct Molecular Applications 211
PART IV Purification of Nucleic Acids from Parasites and Insects
Chapter 12 Isolation of Nucleic Acids from Protozoa 253
Chapter 13 Isolation of Nucleic Acids from Helminthes 275
Chapter 14 Isolation of Nucleic Acids from Insects 297
Chapter 15 Preparation of Parasitic Specimens for Direct Molecular Applications 317
PART V Purification of Nucleic Acids from Mammals
Chapter 16 Isolation of Nucleic Acids from Body Fluids 381
Chapter 17 Isolation of Nucleic Acids from Soft Tissues 405
Chapter 18 Isolation of Nucleic Acids from Hard Tissues 427
Chapter 19 Isolation of DNA from Ancient Samples 449
Chapter 20 Preparation of Forensic Samples for Direct Molecular Applications 471
PART VI Purification of Nucleic Acids from Plants
Chapter 21 Isolation of Nucleic Acids from Plants 503
Chapter 22 Isolation of Megabase-Sized DNA Fragments from Plants 521
PART VII Purification of Nucleic Acids from Miscellaneous Sources
Chapter 23 Purification of Nucleic Acids from Gels 537
Index 555
