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新书资源(2009年11月)

Fluorescence applications in biotechnology and life sciences / edited by Ewa M. Goldys. — Oxford : Wiley-Blackwell, c2009. – (58.1054/F646)

Contents

    CONTENTS
    
    Preface
    Acknowledgments
    About the Contributing Authors
    1 Basics of Fluorescence
    1.1 Introduction
    1.2 Absorption and Emission of Light
    1.3 Nonradiative Decay Mechanisms
    1.4 Properties of Excited Molecules
    1.5 Spectroscopy and Fluorophores
    1.6 Environmental Sensitivity of Fluorophores
    1.7 Polarization of Fluorescence
    1.8 Conclusion
    References
    2 Labeling of Cells with Fluorescent Dyes
    2.1 Introduction
    2.2 Fluorophore Selection
    2.3 Loading and Labeling Live Cells
    2.4 Fluorophores for Live Cell Imaging
    References
    3 Genetically Encoded Fluorescent Probes: Some Properties and Applications in Life Sciences
    3.1 Introduction
    3.2 Chromophore and its Formation
    3.3 "Life and Death" of Fluorescent Protein
    3.4 Applications
    3.5 Passive Applications
    3.6 Active Applications
    3.7 Interactive Applications
    3.8 Conclusions
    References
    4 Nanoparticle Fluorescence Probes
    4.1 Introduction
    4.2 Nanomaterials for Biological Applications
    4.3 Inorganic Quantum Dots: Physics and Optical Properties
    4.4 Synthesis of Monodisperse Colloidal Quantum Dots for Biolabeling Applications
    4.5 Quantum Dots as In Vitro Probes
    4.6 Quantum Dots as In Vivo Probes Cytotoxicity Future Directions References
    5 Quantitative Analysis of Fluorescent Image: From Descriptive to Computational Microscopy
    5.1 Introduction
    5.2 Advantages of Quantitative Analysis
    5.3 Methods of Quantitative Analysis
    5.4 Image Processing
    References
    6 Spectral Imaging and Unmixing
    6.1 Introduction
    6.2 Instrumentation and Configurations for Hyperspectral Microscopes
    6.3 Supervised and Unsupervised Unmixing
    6.4 Supervised or Informed Unmixing
    6.5 Unsupervised or Blind Unmixing
    6.6 Spectral Clustering
    6.7 Examples
    6.8 Limitations of Spectral Imaging and Experimental Considerations
    6.9 Conclusions
    References
    7 Correlation of Light with Electron Microscopy: A Correlative Microscopy Platform
    7.1 Introduction
    7.2 Overview of Techniques Used in Correlative Microscopy
    7.3 Correlative Microscopy of Chemically Fixed, Immunolabeled Ultrathin Cryosections Employing Antibodies Coupled with Fluorescent Gold Conjugates
    7.4 Special Techniques Used in Sample Preparation for Correlative Microscopy: High-Pressure Freezing, Freeze Fracturing, and Freeze Substitution
    7.5 Correlative Microscopy of Fixed Tissue Specimens Using Freeze Substitution
    7.6 Future Trends: Correlative Microscopy and High-Content Cellular Screening
    7.7 Future Trends: Correlative Microscopy of Fluorescent Images Acquired by Confocal Laser Scanning Microscopy
    References
    8 Fluorescence Resonance Energy Transfer and Applications
    8.1 What is FRET?
    8.2 Why FRET Can Be Useful
    8.3 How FRET Can Be Measured
    8.4 Emerging Applications Including Novel FRET Probes
    8.5 Advanced FRET Methods
    References
    9 Monitoring Molecular Dynamics in Live Cells Using Fluorescence Photobleaching
    9.1 Introduction
    9.2 Photobleaching Theory
    9.3 Dynamics of Macromolecules
    9.4 Photobleaching Measurements with Confocal Microscope
    9.5 Photobleaching Applications
    9.6 Conclusions
    References
    10 Time-Resolved Fluorescence in Microscopy
    10.1 Introduction
    10.2 Photophysics and Deactivation of Excited State
    10.3 Time-Resolved Fluorescence Measurements
    10.4 Time-Resolved Fluorescence in Microscopy
    10.5 Conclusions
    References
    11 Fluorescence Correlation Spectroscopy
    11.1 Introduction
    11.2 Optics of Fluorescence Correlation Spectroscopy
    11.3 Practical Aspects of FCS Experiments
    11.4 Quantitative Evaluation of FCS Measurements to Obtain Diffusion Constants and Concentration
    11.5 Conclusion
    References
    12 Flow Cytometry
    12.1 What is Flow Cytometry?
    12.2 Excitation Sources
    12.3 Signal Detection and Analysis
    12.4 Cell Sorting
    12.5 Applications of Flow Cytometry
    References
    13 Fluorescence in Diagnostic Imaging
    13.1 Introduction
    13.2 Principles of Fluorescence Applied to Medical Diagnosis
    13.3 Optical Fluorescence Imaging Techniques In Vivo
    13.4 Imaging of Whole-Body Biological Systems
    13.5 Future Directions
    14 Fluoreseenee in Clinieal Diagnosis
    14.1 Introduction
    14.2 Applications of Fluorescence in Clinical Biochemistry
    14.3 Fluorescence in Pathology and Cancer Diagnostics
    References
    15 Immunochemical Detection of Analytes by Using Fluorescence
    15.1 Introduction: Definition and General Principles of Immunoassays
    15.2 Immunoassay Types and Formats
    15.3 Types of Analytes
    15.4 Steady-State Fluorescence Immunoassays
    15.5 Time-Resolved and Kinetic Approaches as Tools for Elimination of Fluorescence Background
    15.6 Recent Advances in Immunoassay Signal Enhancement and Throughput
    References
    16 Membrane Organization
    16.1 Concepts of Organization of Biological Membranes
    16.2 Fluorescence Methods to Study Membrane Organization
    16.3 Model Membranes
    16.4 Membrane Organization in Cells
    References
    17 Probing Kinetics of Ion Pumps Via Voltage-Sensitive Fluorescent Dyes
    17.1 Introduction: Voltage-Dependent Physiological Processes, Ion Pumps, and Channels
    17.2 Voltage-Sensitive Dyes: Mechanisms, Response Times, and Their Relevance for Kinetic Studies
    17.3 Steady-State Ion Pump Activity
    17.4 Kinetics of Ion Pump Partial Reactions
    17.5 Future Directions
    References
    Index