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新书资源(2009年11月)

Mitochondrial DNA : methods and protocols / edited by Jeffrey A. Stuart. — Dordrecht ; London : Humana Press, c2009. – (58.17/M592/v.554)

Contents

    Contents
    
    Preface
    Contributors
    Part I: MITOCHONDRIAL DNA REPLICATION, TRANSCRIPTION, TRANSLATION, AND RESPIRATORY COMPLEX ASSEMBLY
    1. Biochemical Isolation of mtDNA Nucleoids from Animal Cells.
    2. Analysis of Mitochondrial DNA by Two-Dimensional Agarose Gel Electrophoresis
    3. Comparative Purification Strategies for Drosophila and Human Mitochondrial DNA Replication Proteins: DNA Polymerase γ and Mitochondrial Single-Stranded DNA-Binding Protein
    4. Functional Analysis of Mutant Mitochondrial DNA Polymerase Proteins Involved in Human Disease
    5. Preparation of Human Mitochondrial Single-Stranded DNA-Binding Protein
    6. Methods for Assessing Binding of Mitochondrial Transcription Factor A (TFAM) to DNA
    7. Inducible Expression in Human Cells, Purification, and In Vitro Assays for the Mitochondrial DNA Helicase Twinkle
    8. Purification Strategy for Recombinant Forms of the Human Mitochondrial DNA Helicase
    9. Methods for Studying Mitochondrial Transcription Termination with Isolated Components
    10. Oxidative Phosphorylation: Synthesis of Mitochondrially Encoded Proteins and Assembly of Individual Structural Subunits into Functional Holoenzyme Complexes
    Part II: MITOCHONDRIAL DNA DAMAGE AND REPAIR11. Reactive Oxygen Species Production by Mitochondria.
    12. Measuring mtDNA Damage Using a Supercoiling-Sensitive qPCR Approach
    13. Quantitative Analysis of Oxidized Guanine, 8-Oxoguanine, in Mitochondrial DNA by Immunofluorescence Method 199
    14. In Vitro Measurement of DNA Base Excision Repair in Isolated Mitochondria 213
    15. Targeting Repair Proteins to the Mitochondria of Mammalian Cells Through Stable Transfection, Transient Transfection, Viral Transduction, and TAT-Mediated Protein Transduction 233
    16. Construction and Characterization of a Cell Line Deficient in Repair of Mitochondrial, but Not Nuclear, Oxidative DNA Damage 251
    Part III: MITOCHONDRIAL DNA MUTATIONS
    17. Mitochondrial DNA Oxidative Damage and Mutagenesis in Saccharomyces cerevisiae 267
    18. Determination of DNA Mutation Load in Human Tissues Using Denaturing HPLC-Based Heteroduplex Analysis 287
    19. Rapid Identification of Unknown Heteroplasmic Mitochondrial DNA Mutations with Mismatch-Specific Surveyor Nuclease 301
    20. Collection of Isolated Cells for Studying Mitochondrial DNA Mutations Within Individual Cells 315
    21. Quantitative Analysis of Somatic Mitochondrial DNA Mutations by Single-Cell Single-Molecule PCR 329
    22. Measuring DNA Precursor Pools in Mitochondria
    23. Establishment of Human Cell Lines Lacking Mitochondrial DNA