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新书资源(2009年10月)

Transgenic maize : methods and protocols / edited by M. Paul Scott. — New York : Humana, c2009. – (58.17/M592/v.526)

Contents

    Contents
    
    Contributors
    PART I: INTRODUCTION
    1 Design of Gene Constructs for Transgenic Maize
    PART II: TRANSFORMATION METHODS
    2 Transient Expression of GFP in Immature Seed Tissues
    3 Biolistic Gun-Mediated Maize Genetic Transformation
    4 Agrobacterium-Mediated Maize Transformation: Immature Embryos Versus Callus
    5 Whiskers-Mediated Maize Transfbrmation
    PART III: TRANSGENIC MAIZE IN RESEARCH
    6 Methods for Generation and Analysis of Fluorescent Protein-Tagged Maize Lines
    7 Use of Transgene-Induced RNAi to Regulate Endogenous Gene Expression
    8 Plasmid Rescue: Recovery of Flanking Genomic Sequences from Transgenic Transposon Insertion Sites
    PART IV: ANALYSIS OF TRANSGENIC PLANTS
    9 Nonradioactive Genomic DNA Blots for Detection of Low Abundant Sequences in Transgenic Maize
    10 Tissue-Print Immunodetection of Transgene Products in Endosperm for High-Throughput Screening of Seeds
    11 Determination of Transgene Copy Number by Real-Time Quantitative PCR 129
    PART V: BREEDING WITH TRANSGENES
    12 Herbicide Resistance Screening Assay
    13 Characterizing Transgene Inheritance
    14 Backcross Breeding
    Appendix A
    Appendix B
    Index