Epitope mapping protocols / edited by Ulrich Reineke, Mike Schutkowski. — 2nd ed. — Totowa, N.J. : Humana ; [London : Springer, distributor], c2009. – (58.17/M592/v.524)

Contents

    Contents
    
    Contributors
    SECTION I: B-CELL EPITOPE MAPPING
    1 What Is a B-Cell Epitope?
    A: WHOLE ANTIGEN METHODS
    2 Structural Basis of Antibody-Antigen Interactions
    3 Epitope Mapping of Antibody-Antigen Complexes by Nuclear Magnetic Resonance Spectroscopy
    4 A Solid-Phase Mutual Inhibition Assay with Labeled Antigen
    5 Epitope Mapping by Surfhce Plasmon Resonance
    6 Proteolytic Fragmentation for Epitope Mapping
    7 Epitope Mapping by Proteolysis of Antigen-Antibody Complexes 87
    8 Identifying Residues in Antigenic Determinants by Chemical Modification 103
    9 Epitope Mapping by Diffkrential Chemical Modification of Antigens 119
    B: PEPTIDE LIBRARY APPROACHES
    10 Linear B-Cell Epitope Mapping Using Enzyme-Linked Immunosorbent Assay for Libraries of Overlapping Synthetic Peptides 137
    11 Antibody Epitope Mapping Using SPOTTM Peptide Arrays 145
    12 Peptide Microarrays for Profiling of Modification State-Specific Antibodies 169
    13 Epitope Mapping Using Phage Display Peptide Libraries 181
    14 Antibody Epitope Mapping Using De Novo Generated Synthetic Peptide Libraries 203
    15 Antibody Specificity Profiling on Functional Protein Microarrays 213
    16 Peptide Microarrays for Determination of Cross-Reactivity 225
    C: PROFILING OF ANTIBODY SIGNATURES IN BIOLOGICAL FLUIDS
    17 Epitope Mapping Using Randomly Generated Peptide Libraries 237
    18 Probing the Epitope Signatures of IgG Antibodies in Human Serum from Patients with Autoimmune Disease 247
    19 Microarrayed Allergen Molecules for Diagnostics of Allergy 259
    20 Monitoring B Cell Response to Immunoselected Phage-Displayed Peptides by Microarrays 273
    D: ANTIGEN EXPRESSED FROM RECOMBINANT DNA
    21 Epitope Mapping Using Homolog-Scanning Mutagenesis
    22 Epitope Mapping by Region-Specified PCR-Mutagenesis.
    23 Epitope Mapping Using Phage-Display Random Fragment Libraries
    E: B-CELL EPITOPE PREDICTION
    24 Prediction of Linear B-cell Epitopes
    SECTION II: T-CELL EPITOPE MAPPING
    25 Molecular Recognition of Diverse Ligands by T-Cell Receptors
    26 Identification of Human MHC Class I Binding Peptides using the iTOPIATM Epitope Discovery System 361
    27 T-Cell Epitope Mapping in Mycobacterium tuberculosis Using PepMixes Created by Micro-Scale SPOTTM Synthesis
    28 High-Throughput T-Cell Epitope Discovery Through MHC Peptide Exchange 383
    29 T-Cell Epitope Processing (The Epitope Flanking Regions Matter) 407
    30 Identification of MHC Class II Binding Peptides: Microarray and Soluble MHC Class II Molecules 417
    32 Identification and Validation of T-Cell Epitopes Using the IFN-y EliSpot Assay
    Index 447