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Micro and nano technologies in bioanalysis : methods and protocols / edited by James Weifu Lee and Robert S. Foote. — Dordrecht ; London : Humana Press, c2009. – (58.17/M592/v.544) |
Contents
Contents
Preface
Acknowledgments
Contributors
Part I Applications of Microfluidics and Nanopores in Separation， Detection， Manipulation， and Analysis of Biomolecules
1 HPLC-Chip/MS Technology in Proteomic Profiling
2 Nanofluidic Channel Fabrication and Manipulation of DNA Molecules
3 A Single-Molecule Barcoding System using Nanoslits for DNA Analysis: Nanocoding 29
4 Microfluidic Devices with Photodefinable Pseudo-valves for Protein Separation
5 Microfluidic Chips Designed for Measuring Biomolecules Through a Microbead-Based Quantum Dot Fluorescence Assay
6 DNA Focusing Using Microfabricated Electrode Arrays
7 Solid-State Nanopore for Detecting Individual Biopolymers
8 Inserting and Manipulating DNA in a Nanopore with Optical Tweezers
9 Forming an α-Hemolysin Nanopore for Single-Molecule Analysis 113
10 Nanopore Force Spectroscopy on DNA Duplexes
Part II Technologies of Physical Science and Chemistry in Detection and Analysis of Biomolecules
11 Quantitative Chemical Analysis of Single Cells
12 Trapping and Detection of Single Molecules in Water..
13 ZnO Nanorods as an Intracellular Sensor for pH Measurements
14 Analysis of Biomolecules Using Surface Plasmons
15 Use of Residual Dipolar Couplings in Structural Analysis of Protein-Ligand Complexes by Solution NMR Spectroscopy
16 Raman-Assisted X-Ray Crystallography for the Analysis of Biomolecules 253
17 Methods and Software for Diffuse X-Ray Scattering from Protein Crystals 269
18 Deuterium Labeling for Neutron Structure-Function-Dynamics Aaaalysis 281
19 Small-Angle Neutron Scattering for Molecular Biology: Basics and Instrumentation 293
20 Small-Angle Scattering and Neutron Contrast Variation for Studying Bio-Molecular Complexes 307
21 Protein Sequencing with Tandem Mass Spectrometry
22 Metabolic Analysis
Part III Applications of Quantum Dots and Molecular Fluorescence in Detection， Tracking and Imaging of Biomolecules
23 Multicolor Detection of Combed DNA Molecules Using Quantum Dots 357
24 Quantum Dot Molecular Beacons for DNA Detection 367
25 Quantum Dot Hybrid Gel Blotting: A Technique for Identifying Quantum Dot-Protein/Protein-Protein Interactions 381
26 In Vivo Imaging of Quantum Dots
27 Semiconductor Fluorescent Quantum Dots: Efficient Biolabels in Cancer Diagnostics
28 The Monitoring and Affinity Purification of Proteins Using Dual Tags with Tetracysteine Motifs 421
29 Use of Genomic DNA as Reference in DNA Microarrays
30 Single-Molecule Imaging of Fluorescent Proteins Expressed in Living Cells
31 MicroPET， MicroSPECT， and NIR Fluorescence Imaging of Biomolecules In Vivo
32 Ultrahigh Resolution Imaging of Biomolecules by Fluorescence Photoactivation Localization Microscopy 483
Part IV Nanotechnologies for Biomolecular Delivery， Gene Therapy and Expression Control
33 Real-Time Imaging of Gene Delivery and Expression with DNA Nanoparticle Technologies 525
34 Nanoparticle-Mediated Gene Delivery
35 Magnetic Nanoparticles for Local Drug Delivery Using Magnetic Implants 559
36 Functionalized Magnetic Nanoparticles as an In Vivo Delivery System 571
37 Formulation/Preparation of Functionalized Nanoparticles for In Vivo Targeted Drug Delivery 589
38 Detection of mRNA in Single Living Cells Using AFM Nanoprobes
39 Reverse Transfection Using Gold Nanoparticles
40 Custom-Designed Molecular Scissors for Site-Specific Manipulation of the Plant and Mammalian Genomes 617
41 Determining DNA Sequence Specificity of Natural and Artificial Transcription Factors by Cognate Site Identifier Analysis 637
Index 655
