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Apoptosis : methods and protocols / edited by Peter Erhardt and Ambrus Toth. — 2nd ed. — Dordrecht ; London : Humana Press, c2009. – (58.17/M592/v.559) |
Contents
Contents
Contributors
PART I: DETECTION OF APOPTOSIS
1 Analysing Caspase Activation and Caspase Activity in Apoptotic Cells
2 Flow Cytometry-Based Apoptosis Detection
3 Live to Dead Cell Imaging
4 Detection of Apoptosis in Tissue Sections
5 Detection of Apoptosis in Cell-Free Systems
PART II: DETECTION OF Non-APOPTOTIC CELL DEATH
6 Methods to Analyze Cellular Necroptosis
7 Detection of Cell Death by Autophagy.
PART III: MODIFICATIONS OF APOPTOTIC PROTEINS DURING APOPTOSIS
8 Methods to Analyze Transglutamination of Proteins Involved in Apoptosis 107
9 Methods to Analyze S-nitrosylation of Proteins Involved in Apoptosis
10 Application of In Vivo EPR for Tissue pO2 and Redox Measurements
PART IV: ANALYSIS OF THE FUNCTION OF MAJOR REGULATORS OF APOPTOSIS/CELL SURVIVAL
11 Assays to Measure p53-Dependent and -Independent Apoptosis 143
12 Measurement of Changes in Cdk2 and Cyclin O-Associated Kinase Activity in Apoptosis
13 Fluoromctric Methods for Detection of Mitochondrial Membrane Permeabilization in Apoptosis
14 Regulation of Apoptosis by the Unfolded Protein Response 191
15 Detection of Uncoupling Protein-2 (UCP2) As a Mitochondrial Modulator of Apoptosis
16 Multiple Approach to Analyzing the Role of MicroRNAs in Apoptosis 219
17 Assessment of Apoptotic Cell Phagocytosis by Macrophages
PART V: ANALYSIS OF APOPTOSIS IN DIFFERENT ORGANS
18 Detection of Apoptosis in Mammalian Development 259
19 Detection of Apoptosis in the Central Nervous System 273
20 Genetic Mapping of Anti-Apoptosis Pathways in Myeloid Progenitor Cells 283
21 Analysis of Apoptosis in Isolated Primary Cardiac Myocytes 293
22 Cell Death in Myoblasts and Muscles
PART VI: ANALYSIS OF APOPTOSIS IN MODEL ORGANISMS
23 Reliable Method for Detection of Programmed Cell Death in Yeast 335
24 Detection of Cell Death in Drosophila 343
25 Detecting Apoptotic Cells and Monitoring Their Clearance in the Nematode Caenorhabditis elegans
26 Detection of Herpes Simplex Virus Dependent Apoptosis
Index
